Upregulation of T-bet expression in peripheral blood mononuclear cells during Vogt-Koyanagi-Harada disease
Upregulation of T-bet expression in peripheral blood mononuclear cells during Vogt-Koyanagi-Harada disease
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DOI：
10.1136/bjo.2005.074062
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发表时间：
2005-11-01
影响因子：
4.1
通讯作者：
Kijlstra, A
中科院分区：
文献类型：
--
作者：
Li, B;Yang, P;Kijlstra, A
目的：为了检验这一假设，T-bet的表达是改变患者Vogt-小柳原田（VKH）diseases.Methods：外周血抽取16 VKH患者免疫抑制剂治疗前后和16名健康人。通过ELISA法测定血清和加或不加植物血凝素（PHA）培养的外周血单核细胞（PBMC）上清液中的IFN-γ、IL-2和IL-4。结果：与对照组相比，患者PBMC培养上清中IFN-γ水平明显升高，而IL-2和IL-4水平无明显变化。与对照组相比，VKH患者在活动性葡萄膜炎发作期间发现T-bet mRNA显著增加，但在静止期患者中未发现。T-bet蛋白在活动性葡萄膜炎发作期间的VKH患者中可检测到，但在静止期患者和健康对照中均未检测到。PHA刺激PBMC导致T-bet mRNA和蛋白表达的显着上调的患者和对照组之间没有显着差异两group.Conclusions：上调的T-bet可能与VKH疾病的Th 1介导的免疫反应的发展。
Aim: To test the hypothesis that T-bet expression is altered in patients with Vogt-Koyanagi-Harada (VKH) disease.Methods: Peripheral blood was withdrawn from 16 VKH patients before and after immunosuppressive treatment and from 16 healthy individuals. IFN-gamma, IL-2, and IL-4 in the serum and the supernatants of peripheral blood mononuclear cells (PBMC) cultured with or without phytohaemagglutinin (PHA) were measured by ELISA. T-bet mRNA and protein expression in PBMC cultured with or without PHA was detected by RT-PCR and western blot, respectively.Results: The level of IFN-gamma, but not IL-2 and IL-4, was significantly higher in the supernatants of stimulated PBMC in patients than in controls. A significantly increased T-bet mRNA was found in VKH patients during an active uveitis episode, but not in quiescent patients, compared to controls. T-bet protein was detectable in VKH patients during an active uveitis episode, but not in quiescent patients nor in the healthy controls. Stimulation of PBMC with PHA resulted in a marked upregulation of T-bet mRNA and protein expression for both patients and controls with no significant difference between the two groups.Conclusions: Upregulation of T-bet may be associated with the development of a Th1 mediated immune response in VKH disease.