The cDNA cloning and ontogeny of mouse α-synuclein
The cDNA cloning and ontogeny of mouse α-synuclein
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DOI：
10.1097/00001756-199804200-00051
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发表时间：
1998-04-20
期刊：
影响因子：
1.7
通讯作者：
Suh, YH
中科院分区：
文献类型：
--
作者：
Hong, L;Ko, HW;Suh, YH
α - synuclein与帕金森病的发病机制有关。为了研究α -突触核蛋白在脑中的作用，我们从小鼠脑cDNA文库中分离了人类α -突触核蛋白的小鼠同源基因cDNA克隆。开放阅读框编码140个氨基酸，与人类α -突触核蛋白有95%的同源性。Northern blot分析显示，α -突触核蛋白mRNA主要在成年小鼠脑和脾脏中表达。原位杂交组织化学显示α -突触核蛋白mRNA在成年小鼠海马组织和新皮层的表达最高。α -突触核蛋白mRNA在出生后第1天首次在海马和新皮层中表达。这些前脑区α -突触核蛋白mRNA的水平在出生后第7天几乎达到最高，并一直保持相对较高的水平直到成虫期。α -突触核蛋白mRNA在胚胎发生过程中在肝脏中短暂表达。(C) 1998快速科学有限公司
ALPHA-SYNUCLEIN has been implicated in the pathogenesis of Parkinson's disease. To investigate the role of alpha-synuclein in the brain, the cDNA clone encoding the mouse cognate of the human alpha-synuclein was isolated from a mouse brain cDNA library. The open reading frame coded for 140 amino acids that share 95% identity with human alpha-synuclein. Northern blot analysis showed that alpha-synuclein mRNA was primarily expressed in brain and spleen of adult mouse. In situ hybridization histochemistry revealed the highest expression of alpha-synuclein mRNA in the hippocampal formation and neocortex of the adult mouse. alpha-Synuclein mRNA expression in the brain was first observed in the hippocampus and neocortex on postnatal day 1. Levels of alpha-synuclein mRNA in these forebrain areas were nearly maximal at postnatal day 7 and remained relatively high until the adult stage. alpha-Synuclein mRNA was expressed in the liver transiently during embryogenesis. (C) 1998 Rapid Science Ltd.