VACUOLAR PH REGULATION IN CHARA-AUSTRALIS
VACUOLAR PH REGULATION IN CHARA-AUSTRALIS
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DOI：
10.1247/csf.9.225
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发表时间：
1984-01-01
影响因子：
1.5
通讯作者：
TAZAWA, M
中科院分区：
文献类型：
--
作者：
MORIYASU, Y;SHIMMEN, T;TAZAWA, M
通过液泡灌流C.澳大利亚。灌注后，将细胞在人工池水中孵育。在不同的孵育时间后分离细胞汁液，并用玻璃pH微电极测量其pH。用细胞内pH敏感的锑电极原位测量pH变化的时间过程。当用pH6.0的人工细胞液替换天然细胞液（pH 5）时，pH逐渐恢复到5。该恢复过程被DCCD [二环己基碳二亚胺]和H+-ATP酶抑制剂抑制。当用pH4.2的人工细胞液替换天然细胞液时，pH迅速向碱性方向移动（达到pH 6，之后返回到原始值，pH 5）或直接返回到原始值。这一过程不受DCCD的抑制。显然，pH值是由液泡膜的H+泵提供的H+主动流入液泡和H+被动流出液泡之间的平衡来调节的。
The vacuolar pH (pHv) was modified by vacuolar perfusion of internodal cells of C. australis. After perfusion, the cell was incubated in artificial pond water. The cell sap was isolated after various incubation periods and its pH was measured with a glass pH microelectrode. The time course of pHv change also was measured in situ with an intracellular pH-sensitive antimony electrode. When the natural cell sap (pH 5) was replaced with artifical cell sap of pH 6.0, the pH gradually returned to 5. This recovery process was inhibited by DCCD [dicyclohexylcarbodiimide], and inhibitor of H+-ATPase. When the natural cell sap was replaced with an artifical cell sap of pH 4.2, the pH quickly shifted to the alkaline direction (reaching a pH of 6, after which it returned to the original value, pH 5) or it returned directly to the original value. This process was not inhibited by DCCD. Apparently the pHv is regulated by the balance between the active influx of H+ into the vacuole provided by the H+-pump of the tonoplast and the passive efflux of H+ from the vacuole.