The human fetal omentum: a site of B cell generation.
The human fetal omentum: a site of B cell generation.
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人类胎儿大脑：B细胞产生的位置。
DOI：
10.1084/jem.175.2.397
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发表时间：
1992-02-01
期刊：
影响因子：
--
通讯作者：
Kearney JF
中科院分区：
文献类型：
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作者：
Solvason N;Kearney JF
胎鼠网膜已被证明是前体细胞的来源，其仅重建Ly 1 + B细胞和密切相关的Ly 1-姐妹细胞群，而不是常规的B细胞或T细胞。我们已经扩展了这些研究，以比较B细胞在人类胎儿网膜，肝脏和脾脏的发展，并证明了前/前B细胞室（CD 24+，sIgM-）检测到网膜和肝脏，但不是脾脏早在8周的妊娠。从妊娠8至12周，网膜和肝脏中IgM+前B细胞（cIgM+/sIgM-）的比例分别为53 +/- 15%和45 +/-13%，脾脏中未检测到IgM+细胞。12周后，胎儿肝脏中的前B细胞百分比不变（41 +/- 10%），但网膜中的前B细胞百分比显著降低（25 +/- 14%）;现在在脾脏中检测到前B细胞，但百分比（2 +/- 3%）远低于网膜或肝脏。在8-12周的时间段内，在网膜和肝脏中约25%的CD 24+细胞中检测到核酶Tdt，然而，在脾脏中未检测到Tdt+细胞。在网膜和脾脏中发现的成熟B细胞中约有40%为CD 5+，而在肝脏中仅为20%。这些结果表明，胎儿大网膜与胎儿肝脏和骨髓一样，是B细胞发育的主要部位。
The fetal mouse omentum has been shown to be a source of precursors that exclusively reconstitutes Ly1+ B cells and the closely related Ly1- sister population, but not conventional B cells or T cells. We have extended these studies to compare B cell development in the human fetal omentum, liver, and spleen, and to demonstrate that the pro/pre-B cell compartment (CD24+, sIgM-) is detected in the omentum and liver but not spleen as early as 8 wk of gestation. From 8 to 12 wk of gestation, the proportions of IgM+ cells that were pre-B cells (cIgM+/sIgM-) in the omentum and liver were 53 +/- 15% and 45 +/- 13%, respectively, and IgM+ cells were not detectable in the spleen. After 12 wk, the percentage of pre-B cells was unchanged in the fetal liver (41 +/- 10%) but decreased significantly in the omentum (25 +/- 14%); pre-B cells were now detected in the spleen but at much lower percentages (2 +/- 3%) than either the omentum or liver. The nuclear enzyme, Tdt, was detected in approximately 25% of the CD24+ cells in the omentum and liver during the 8-12-wk time period, however, Tdt+ cells were not detected in the spleen. Approximately 40% of the mature B cells found in the omentum and spleen were CD5+ compared with only 20% in the liver. These results demonstrate that the fetal omentum, like the fetal liver and bone marrow, is a primary site of B cell development.