A High Fat Diet Increases Bone Marrow Adipose Tissue (MAT) But Does Not Alter Trabecular or Cortical Bone Mass in C57BL/6J Mice.
A High Fat Diet Increases Bone Marrow Adipose Tissue (MAT) But Does Not Alter Trabecular or Cortical Bone Mass in C57BL/6J Mice.
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DOI：
10.1002/jcp.24954
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发表时间：
2015-09
影响因子：
5.6
通讯作者：
Rosen CJ
中科院分区：
文献类型：
--
作者：
Doucette CR;Horowitz MC;Berry R;MacDougald OA;Anunciado-Koza R;Koza RA;Rosen CJ
肥胖与高骨密度（BMD）有关，但更容易骨折。一些肥胖者骨髓脂肪组织（MAT）增加，但MAT对骨转换的影响仍然存在争议，高脂肪饮食（HFD）引起的骨密度变化也是如此。在这项研究中，我们假设MAT体积会对HFD产生反应，但与BMD的变化无关。因此，我们在3周龄时给C57BL/6J （B6）雄性小鼠喂食高脂饮食（60 kcal %）或普通饮食（10 kcal %） 12周（n=10/组）。采用锇染色法和微ct （micro-CT）法测定MAT体积，采用μCT法、组织形态学法和双能x线吸收法测定骨参数。我们还进行了一项短期试点研究，使用13周龄的B6雄性和雌性饲喂HFD(58千卡%)2周（n=3/性别）。长期和短期喂食HFD均与高MAT体积相关，但在长期研究中，股骨小梁骨体积分数（BV/TV）、骨形成率和皮质骨量没有改变。在短期的初步研究中，两周的HFD后，实际骨密度没有变化。我们得出的结论是，对于从断奶或13周龄开始饲喂HFD的B6小鼠，MAT增加，而骨量没有改变。需要更多的研究来确定骨髓中能量快速储存的机制，以及它与其他脂肪储存的区别。
Obesity has been associated with high bone mineral density (BMD) but a greater propensity to fracture. Some obese individuals have increased marrow adipose tissue (MAT), but the impact of MAT on bone turnover remains controversial, as do changes in BMD associated with a high fat diet (HFD). In this study we hypothesized that MAT volume would increase in response to HFD but would be independent of changes in BMD. Hence, we fed C57BL/6J (B6) male mice at 3 weeks of age either a high fat diet (60 kcal %) or regular diet (10 kcal %) for 12 weeks (n=10/group). We measured MAT volume by osmium staining and micro-CT (μCT) as well as bone parameters by μCT, histomorphometry, and dual-energy X-ray absorptiometry. We also performed a short-term pilot study using 13-week-old B6 males and females fed a HFD (58 kcal %) for 2 weeks (n=3/sex). Both long- and short-term HFD feedings were associated with high MAT volume, however, femoral trabecular bone volume fraction (BV/TV), bone formation rate and cortical bone mass were not altered in the long-term study. In the short-term pilot study, areal BMD was unchanged after two weeks of HFD. We conclude that, for B6 mice fed a HFD starting at wean or 13 weeks of age, MAT increases whereas bone mass is not altered. More studies are needed to define the mechanism responsible for the rapid storage of energy in the marrow and its distinction from other adipose depots.