Mapping the early development of projections from the entorhinal cortex in the embryonic mouse using prenatal surgery techniques.
Mapping the early development of projections from the entorhinal cortex in the embryonic mouse using prenatal surgery techniques.
复制标题
使用产前手术技术绘制胚胎小鼠内嗅皮层投射的早期发育图。
DOI：
10.1002/neu.480220903
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Ide,CF
中科院分区：
文献类型：
--
作者：
Snyder,DC;Coltman,BW;Muneoka,K;Ide,CF
这项工作的目的是研究小鼠妊娠晚期后外侧皮质特异性投射的发育。为了做到这一点，我们用荧光碳菁染料Dil标记未分化的外侧皮质，在胚胎（E）16天的小鼠胚胎中使用子宫外手术技术（Muneoka，Wanek和Bryant，1986）。允许胚胎发育至足月（出生后第0天，P0），此时研究了从标记区域发出的纤维模式。将染料放置在未分化的后腹侧皮质中，在海马结构中产生标记的纤维。一个强大的投射到海马CA1区的角束被大量标记。此外，在一些动物中，标记了皮质束，如前连合、胼胝体和皮质顶盖束。这些神经束以前曾被描述为猫胎（McConnell，Ghosh和Shatz，1989）和其他脊椎动物（Wilson，Ross，Parrett和Easter，1990）中的支架通路。染料放置在相邻的，更前面或背侧的地区表现出强烈的标记在皮质结构，但没有标记在海马结构。这些数据表明，出生时，颞叶皮层沿沿着喙尾轴细分为内嗅皮层和嗅周皮层，沿沿着背腹轴细分为内嗅皮层和新皮层。此外，这些最早期的连接在目标区域选择的特异性方面与成人连接相似。因此，这些早期但特定的连接可能在出生后发育期间形成未来连接中发挥作用。
The purpose of this work was to study the development of specific projections from the postero‐lateral cortex during the third trimester of gestation in the mouse. To do this, we labeled undifferentiated lateral cortex with the fluorescent carbocyanine dye, Dil, in the embryonic day (E) 16 mouse embryo usingexo uterosurgical techniques (Muneoka, Wanek, and Bryant, 1986). Embryos were allowed to develop to term (postnatal day 0, P0) at which time the fiber patterns emanating from the marked regions were studied. Dye placement in the undifferentiated postero‐ventral cortex produced labeled fibers in the hippocampal formation. A robust projection of the angular bundle into the CA1 region of the hippocampus was heavily labeled. In addition, in some animals, cortical tracts, such as the anterior commissure, corpus callosum, and a corticotectal tract, were labeled. These tracts have been described previously as scaffolding pathways in the fetal cat (McConnell, Ghosh, and Shatz, 1989), and other vertebrates (Wilson, Ross, Parrett, and Easter, 1990). Dye placement in adjacent, more anterior or dorsal areas showed strong labeling in cortical structures but no labeling in the hippocampal formation. These data indicate that, by birth, the temporal cortex is subdivided along the rostro‐caudal axis as entorhinal cortex and perirhinal cortex, and along the dorso‐ventral axis, as entorhinal cortex and neocortex. Also, these earliest connections are similar to adult connections in their specificity of target area selection. Therefore, these early, yet specific, connections may play a role in the formation of future connections during postnatal development.