SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III
SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III
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DOI：
10.1101/gad.12.17.2664
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发表时间：
1998-09-01
影响因子：
10.5
通讯作者：
Hernandez, N
中科院分区：
文献类型：
--
作者：
Henry, RW;Mittal, V;Hernandez, N
基础转录因子SNAP（c）与PSE结合，PSE是RNA聚合酶II和III人类snRNA启动子中的焦炭元件。SNAP（c）至少含有四个亚基，但还不可能组装出完全确定的重组SNAP（c）。在这里，我们从五个重组亚基SNAP 43，SNAP 45，SNAP 50，SNAP 190和一个新鉴定的亚基SNAP 19重组SNAP（c）。该重组复合物特异性结合PSE并指导RNA聚合酶II和III snRNA基因转录。因此，相同的核心SNAP（c）使两类起始复合物的组装成核。
The basal transcription factor SNAP(c) binds to the PSE, a coke element in the RNA polymerase II and III human snRNA promoters. SNAP(c) contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAP(c). Here we reconstitute SNAP(c) from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAP(c) nucleates the assembly of two classes of initiation complexes.