The odyssey of a regulated transcript.
The odyssey of a regulated transcript.
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DOI：
10.1017/s135583820000145x
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发表时间：
2000-12
期刊：
影响因子：
4.5
通讯作者：
J. Vilardell;P. Chartrand;R. Singer;J. R. Warner
中科院分区：
文献类型：
--
作者：
J. Vilardell;P. Chartrand;R. Singer;J. R. Warner
酿酒酵母基因RPL30的转录本受到调控剪接和调控翻译的影响，因为它的结构与其自身的产物核糖体蛋白L30相互作用。我们在体内跟踪了受调控的RPL30转录本的命运。最初，这些转录本流产地进入剪接途径，与U1 SnRNP形成了一种异常稳定的结合。然而，很大一部分未剪接的分子存在于细胞质中。其中大多数仍然受到L30的约束，因为只有一小部分人从事翻译。最终，未剪接的RPL30转录本逃脱了L30的控制，与核糖体结合，成为无意义介导的衰退的牺牲品。
The transcript of the Saccharomyces cerevisiae gene, RPL30, is subject to regulated splicing and regulated translation, due to a structure that interacts with its own product, ribosomal protein L30. We have followed the fate of the regulated RPL30 transcripts in vivo. Initially, these transcripts abortively enter the splicing pathway, forming an unusually stable association with U1 snRNP. A large proportion of the unspliced molecules, however, are found in the cytoplasm. Most of these are still bound by L30, as only a small fraction are engaged in translation. Eventually, the unspliced RPL30 transcripts escape the grasp of L30, associate with ribosomes, and fall prey to nonsense mediated decay.