Influence of mechanical tail-detachment techniques of schistosome cercariae on the production, viability, and infectivity of resultant schistosomula: a comparative study
Influence of mechanical tail-detachment techniques of schistosome cercariae on the production, viability, and infectivity of resultant schistosomula: a comparative study
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DOI：
10.1007/s004360000199
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发表时间：
2000-07-01
影响因子：
2
通讯作者：
Flescher, E
中科院分区：
文献类型：
--
作者：
Gold, D;Flescher, E
对曼氏血吸虫尾蚴体外转化生产血吸虫的两种蜕变方法进行了比较试验。尾蚴的低压注射器通过和涡流混合都产生了具有相似活力百分比的血吸虫，并且小鼠皮下注射后的蠕虫恢复水平也相当，尽管较低。根据所有测试标准，包括简单性和时间要求，这两种方法同样有效，并且非常适用于血吸虫的大规模生产。
Two decaudation methods used by Schistosoma mansoni cercariae for the production of schistosomula by in vitro transformation were comparatively tested. Both low-pressure syringe passage and vortex mixing of the cercariae produced schistosomula of similar percentages of viability, and the levels of worm recovery following subcutaneous injection in mice were also comparable, though low. By all criteria tested, including simplicity and time requirement, the two methods are equally efficient and are well applicable for mass production of schistosomula.