The Comparative Invasiveness of Endometriotic Cell Lines to Breast and Endometrial Cancer Cell Lines.
The Comparative Invasiveness of Endometriotic Cell Lines to Breast and Endometrial Cancer Cell Lines.
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DOI：
10.3390/biom13061003
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发表时间：
2023-06-17
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
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作者：
子宫内膜异位症是一种侵袭性疾病，影响全球10%的女性(以及出生时被指定为女性的人)。本研究的目的是研究三种子宫内膜异位症细胞系(EEC12Z、IEC-ESCs、tHESCs)对癌细胞株(MDA-MB-231、SW1353和EM-E6/E7/TERT)的相对侵袭力，并评估不同侵袭试验之间的相对侵袭力是否一致。对所有细胞株进行Transwell、球形下降和球形凝胶侵袭实验，并对波形蛋白、细胞角蛋白、E-钙粘附素和N-钙粘附素进行染色，以评估其表达的变化。在所有检测中，子宫内膜异位症细胞株显示出与本研究中使用的癌细胞株相似的侵袭性，在确定的侵袭性方面没有显著差异。在检测期间已经侵袭的EEC12Z细胞与在检测期间内未侵袭的细胞相比，E-钙粘附素的表达下降，而N-钙粘附素的表达没有明显变化，这可能支持这样的假设，即上皮向间充质的转变影响了该腹膜子宫内膜异位症细胞株的侵袭性。
Endometriosis is an invasive condition that affects 10% of women (and people assigned as female at birth) worldwide. The purpose of this study was to characterize the relative invasiveness of three available endometriotic cell lines (EEC12Z, iEc-ESCs, tHESCs) to cancer cell lines (MDA-MB-231, SW1353 and EM-E6/E7/TERT) and assess whether the relative invasiveness was consistent across different invasion assays. All cell lines were subjected to transwell, spheroid drop, and spheroid-gel invasion assays, and stained for vimentin, cytokeratin, E-Cadherin and N-Cadherin to assess changes in expression. In all assays, endometriotic cell lines showed comparable invasiveness to the cancer cell lines used in this study, with no significant differences in invasiveness identified. EEC12Z cells that had invaded within the assay periods showed declines in E-Cadherin expression compared to cells that had not invaded within the assay period, without significant changes in N-Cadherin expression, which may support the hypothesis that an epithelial-to-mesenchymal transition is an influence on the invasiveness shown by this peritoneal endometriosis cell line.
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影响因子：
4
作者：
Brueggmann D;Templeman C;Starzinski-Powitz A;Rao NP;Gayther SA;Lawrenson K
通讯作者：
Lawrenson K
影响因子：
--
作者：
Amaro A;Angelini G;Mirisola V;Esposito AI;Reverberi D;Matis S;Maffei M;Giaretti W;Viale M;Gangemi R;Emionite L;Astigiano S;Cilli M;Bachmeier BE;Killian PH;Albini A;Pfeffer U
通讯作者：
Pfeffer U
影响因子：
1.8
作者：
De Joliniere, Jean Bouquet;Ayoubi, Jean Marc Bernard;Feki, Anis
通讯作者：
Feki, Anis
影响因子：
3.6
作者：
Aslan M;Hsu EC;Liu S;Stoyanova T
通讯作者：
Stoyanova T
DOI：
10.1083/jcb.200611141
发表时间：
2007-04-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cumming G;Fidler F;Vaux DL
通讯作者：
Vaux DL