Characterization of the major superoxide dismutase of Staphylococcus aureus and its role in starvation survival, stress resistance, and pathogenicity
Characterization of the major superoxide dismutase of Staphylococcus aureus and its role in starvation survival, stress resistance, and pathogenicity
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DOI：
10.1128/jb.181.13.3898-3903.1999
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发表时间：
1999-07-01
影响因子：
3.2
通讯作者：
Foster, SJ
中科院分区：
文献类型：
--
作者：
Clements, MO;Watson, SP;Foster, SJ
金黄色葡萄球菌的突变体（SPW 1），这是无法生存的长期饥饿被证明有一个转座子插入内的基因同源的sodA家族的锰依赖性超氧化物歧化酶（SOD）。亲本8325-4菌株的全细胞裂解物经非变性凝胶电泳显示出三个SOD活性区带，其中两个区带的活性依赖于锰的活性，而SPW 1中不存在。SOD活性和sodA表达水平呈生长期依赖性，主要发生在指数后阶段。这种反应也取决于培养物的通气水平，仅在高通气下发生最高活性和表达。sodA的表达，因此，SOD活性可以诱导甲基紫精，但只有在过渡期从指数到指数后阶段的增长。SPW 1在氨基酸限制和酸胁迫下存活的能力较弱，但与亲本菌株8325-4相比，在小鼠脓肿感染模型中的致病性没有改变。
A Staphylococcus aureus mutant (SPW1) which is unable to survive long-term starvation was shown to have a transposon insertion within a gene homologous to the sodA family of manganese-dependent superoxide dismutases (SOD). Whole-cell lysates of the parental 8325-4 strain demonstrated three zones of SOD activity by nondenaturing gel electrophoresis, The activities of two of these zones were dependent on manganese for activity and were absent in SPW1. The levels of SOD activity and sodA expression were growth-phase dependent, occurring most during postexponential phase. This response was also dependent on the level of aeration of the culture, with highest activity and expression occurring only under high aeration. Expression of sodA and, consequently, SOD activity could be induced by methyl viologen but only during the transition from exponential- to postexponential-phase growth. SPW1 was less able to survive amino acid limitation and acid stress but showed no alteration in pathogenicity in a mouse abscess model of infection compared to the parental strain 8325-4.