Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns.
Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns.
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DOI：
10.1016/j.aca.2014.09.006
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发表时间：
2014-12
影响因子：
6.2
通讯作者：
Jing Han;Linquan Ye;Lin-Tao Xu;Zhuoheng Zhou;F. Gao;Zhiliang Xiao;Qiuquan Wang;Bo Zhang
中科院分区：
文献类型：
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作者：
Jing Han;Linquan Ye;Lin-Tao Xu;Zhuoheng Zhou;F. Gao;Zhiliang Xiao;Qiuquan Wang;Bo Zhang
单次蛋白质组学是一种有前途的高通量蛋白质组学分析方法。在该策略中，需要长毛细管柱来执行长且浅的梯度，以实现高峰容量和良好的峰宽度，从而进行信息丰富的质谱检测。在此，我们报告了可以基于单颗粒熔结技术轻松制造填充有 5 μm 颗粒材料的米长毛细管柱。长柱可以在 10 小时长梯度中可靠地产生 800 的高峰容量，用于蛋白质消化分离。该操作在最广泛使用的常压 nanoLC 系统的压力范围 (40 MPa) 内进行。由于出色的使用寿命（> 100 次进样）和柱间性能一致性，本文报道的米长毛细管柱在单次蛋白质组学中应该具有实际用途，而无需超高压仪器。
Single shot proteomics is a promising approach to high throughput proteomics analysis. In this strategy, long capillary columns are needed to perform long and shallow gradients to achieve high peak capacity and good peak width for informative mass spectrometric detection. Herein, we report that meter long capillary columns, packed with 5 μm particulate material, can be facilely fabricated based on single particle fritting technology. The long columns could reliably generate high peak capacities of 800 in 10 h long gradients for protein digest separations. The operation was within the pressure range (40 MPa) of the most widely used normal pressure nanoLC systems. Due to the excellent life time (>100 injections) and inter-column performance consistency, the meter long capillary columns reported here should be of practical usefulness in single shot proteomics without the need for ultra-high pressure instrumentation.