Glutamate as a putative neurotransmitter in the buccal central pattern generator of Helisoma trivolvis.
Glutamate as a putative neurotransmitter in the buccal central pattern generator of Helisoma trivolvis.
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谷氨酸作为 Helisoma trivolvis 颊中枢模式发生器中假定的神经递质。
DOI：
10.1152/jn.1991.66.4.1264
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发表时间：
1991
影响因子：
2.5
通讯作者：
Murphy,AD
中科院分区：
文献类型：
--
作者：
Quinlan,EM;Murphy,AD
1.研究了L-谷氨酸灌流对三卷Helisoma trivolvis颊神经节内已鉴定神经元的影响。谷氨酸反映了三联摄食中枢模式发生器（CPG）亚单位2（S2）的活性对S2突触后神经元的影响。被S2兴奋的神经元被谷氨酸去极化，而被S2抑制的神经元被谷氨酸超极化。谷氨酸也刺激节律性S2活动。2.不同的谷氨酸受体激动剂可以模拟谷氨酸对颊神经元作用的特定成分。红藻氨酸在接受S2兴奋性突触后电位（EPSP）的神经元中产生去极化，并激活节律性S2活动。使君子酸在接受S2抑制性突触后电位（IPSP）的神经元中产生超极化。3.非N-甲基-D-天冬氨酸谷氨酸受体拮抗剂氰基-7-硝基喹喔啉-2，3-二酮（CNQX）可阻断谷氨酸和红藻氨酸引起的S2 EPSPs和去极化效应，但对谷氨酸和使君子酸引起的S2 IPSPs和超极化效应无效。4.这些数据支持这一假设，谷氨酸是在Helisoma中的喂食CPG的S2的递质，作用于兴奋性突触的CNQX敏感的红藻氨酸样受体和抑制性突触的CNQX不敏感的使君子酸样受体。
1. The effects of L-glutamate superfusion over identified neurons within the buccal ganglia of Helisoma trivolvis were examined. Glutamate mirrored the effect of activity of subunit 2 (S2) of the tripartite feeding central pattern generator (CPG) on S2 postsynaptic neurons. Neurons that are excited by S2 are depolarized by glutamate, whereas neurons that are inhibited by S2 are hyperpolarized by glutamate. Glutamate also stimulated rhythmic S2 activity. 2. Different glutamate agonists could mimic specific components of the effects of glutamate on buccal neurons. Kainate produced depolarizations in neurons that receive S2 excitatory postsynaptic potentials (EPSPs) and activated rhythmic S2 activity. Quisqualate produced hyperpolarizations in neurons that receive S2 inhibitory postsynaptic potentials (IPSPs). 3. The non-N-methyl-D-aspartate glutamate receptor antagonist cyano-7-nitroquinoxaline-2,3-dione (CNQX) blocked the effects of S2 EPSPs and depolarizations produced by application of glutamate and kainate, but was ineffective in blocking S2 IPSPs or hyperpolarizations produced by application of glutamate and quisqualate. 4. These data support the hypothesis that glutamate is the transmitter of S2 of the feeding CPG in Helisoma, acting at CNQX-sensitive kainate-like receptors at excitatory synapses and CNQX-insensitive quisqualate-like receptors at inhibitory synapses.