Role of intrinsic properties in Drosophila motoneuron recruitment during fictive crawling.
Role of intrinsic properties in Drosophila motoneuron recruitment during fictive crawling.
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内在特性在虚构爬行过程中果蝇运动神经元招募中的作用。
DOI：
10.1152/jn.00298.2010
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发表时间：
2010
影响因子：
2.5
通讯作者：
Levine,RichardB
中科院分区：
文献类型：
--
作者：
Schaefer,JenniferE;Worrell,JasonW;Levine,RichardB
大多数生物体中的运动神经元都分为低阈值和高阈值类型，负责产生强大而精确的运动。根据在神经肌肉接头处获得的数据，果蝇 1b 和 1s 运动神经元可能分别类似于低阈值和高阈值神经元，尽管关于行为过程中内在特性或招募的信息很少。因此，使用原位全细胞膜片钳记录来比较果蝇幼虫中 1b 和 1s 运动神经元的参数。我们发现静息膜电位、电压阈值和尖峰延迟可区分 1b 和 1s 运动神经元。 1s 运动神经元中较长的尖峰延迟是 theshal 编码的 A 型 K+ 电流的结果。 1b 和 1s 运动神经元之间的功能差异与行为相关，因为在虚构运动期间突触诱发活动的成对全细胞记录中，MNISN-1 中观察到更高的阈值和更长的峰值延迟。
Motoneurons in most organisms conserve a division into low-threshold and high-threshold types that are responsible for generating powerful and precise movements. Drosophila 1b and 1s motoneurons may be analogous to low-threshold and high-threshold neurons, respectively, based on data obtained at the neuromuscular junction, although there is little information available on intrinsic properties or recruitment during behavior. Therefore in situ whole cell patch-clamp recordings were used to compare parameters of 1b and 1s motoneurons in Drosophila larvae. We find that resting membrane potential, voltage threshold, and delay-to-spike distinguish 1b from 1s motoneurons. The longer delay-to-spike in 1s motoneurons is a result of theshal-encoded A-type K+current. Functional differences between 1b and 1s motoneurons are behaviorally relevant because a higher threshold and longer delay-to-spike are observed in MNISN-1s in pairwise whole cell recordings of synaptically evoked activity during bouts of fictive locomotion.