Effect of various growth media upon survival during storage of freeze-dried Enterococcus faecalis and Enterococcus durans
Effect of various growth media upon survival during storage of freeze-dried Enterococcus faecalis and Enterococcus durans
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DOI：
10.1046/j.1365-2672.2003.01853.x
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发表时间：
2003-01-01
影响因子：
4
通讯作者：
Gibbs, P
中科院分区：
文献类型：
--
作者：
Carvalho, AS;Silva, J;Gibbs, P
目的：研究了MRS、M17和Lee’s三种不同培养基对六株粪肠球菌和两株大肠杆菌在冷冻干燥和随后保存过程中存活率的影响。方法和结果：分离肠球菌属。使菌株在M17、MRS和Lee氏肉汤上生长，冷冻干燥并在20 ℃下避光保存。在整个储存过程中，定期对冻干样品进行复水，然后将其铺在M17琼脂上。在MRS中生长时，获得了durans。然而，对于大多数E.粪肠球菌菌株，这显然是更好地生存在干燥状态时，这种生物体已在M17或李氏培养基中生长。在储存期间测试的结果显示是菌株特异性的，并且依赖于生长培养基。
Aims: The effects of three different growth media (MRS, M17 and Lee's) on survival during freeze-drying and subsequent storage of six strains of Enterococcus faecalis and two strains of E. durans were investigated.Methods and Results: Distinct Enterococcus spp. strains were grown on M17, MRS and Lee's broth, freeze-dried and stored at 20degreesC in air under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and then plated on M17 agar.Conclusions: A higher survival rate during storage of dried E. durans was obtained when growth occurred in MRS. The same effect was not observed, however, for the majority of E. faecalis strains, which clearly survived better in the dried state when this organism had been grown in M17 or Lee's medium.Significance and Impact of Study: The survival of the dried Enterococcus spp. tested during storage was shown to be strain-specific and dependent on the growth medium.