Expression levels and association of gelatinases MMP-2 and MMP-9 and collagenases MMP-1 and MMP-13 with VEGF in synovial fluid of patients with arthritis
Expression levels and association of gelatinases MMP-2 and MMP-9 and collagenases MMP-1 and MMP-13 with VEGF in synovial fluid of patients with arthritis
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DOI：
10.1007/s00296-010-1592-1
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发表时间：
2011-04-01
影响因子：
4
通讯作者：
Yoo, Myung Chul
中科院分区：
文献类型：
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作者：
Kim, Kyoung Soo;Choi, Hyun Mi;Yoo, Myung Chul
进行这项研究是为了间接提供证据，证明明胶酶MMP-2和MMP-9以及胶原酶MMP-1和MMP-13中哪些基质金属蛋白酶（MMPs）在血管生成过程中发挥更积极的作用。关节炎关节。收集33例类风湿性关节炎（RA）和骨关节炎（OA）患者关节液，采用ELISA法检测关节液中MMPs和VEGF的含量，并分析其相关性。RA关节液中胶原酶（总MMP-1和总MMP-13）和明胶酶（总MMP-2和总MMP-9）的水平显著高于OA关节液。总MMP-9水平与RA液体中的VEGF水平显著相关，但在OA液体中不相关，而总MMP-13水平与RA和OA液体中的VEGF水平密切相关。然而，总MMP-2和总MMP-1水平与RA或OA关节液中的VEGF水平无关。我们的研究结果间接表明，在RA和OA，MMP-9和MMP-13可能发挥更重要的作用，在血管生成比MMP-2和MMP-1。
This study was performed to provide evidence, albeit indirectly, as to which matrix metalloproteinases (MMPs), among the gelatinases MMP-2 and MMP-9 and the collagenases MMP-1 and MMP-13, play a more proactive role in the angiogenic process in arthritic joint. Joint fluid was collected from 33 patients with rhuematoid arthritis (RA) and osteoarthritis (OA), and protein (MMPs and vascular endothelial growth factor (VEGF)) levels were measured by ELISA, and the association of MMPs with VEGF was evaluated in joint fluid of patients with RA or OA. The levels of collagenases (total MMP-1 and total MMP-13) and gelatinases (total MMP-2 and total MMP-9) in RA joint fluid were significantly higher than those in OA fluid. Total MMP-9 levels were significantly associated with VEGF levels in RA fluids, but not in OA fluid, while total MMP-13 levels were strongly associated with VEGF levels in both RA and OA fluid. However, total MMP-2 and total MMP-1 levels were not associated with VEGF levels in either RA or OA joint fluid. Our results indirectly suggest that in RA and OA, MMP-9 and MMP-13 may play a more important role in angiogenesis than MMP-2 and MMP-1.