ISOLATION AND CHARACTERIZATION OF A CDNA FOR RAT-LIVER CYSTEINE DIOXYGENASE
ISOLATION AND CHARACTERIZATION OF A CDNA FOR RAT-LIVER CYSTEINE DIOXYGENASE
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DOI：
10.1016/0006-291x(90)92345-z
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发表时间：
1990-04-30
影响因子：
3.1
通讯作者：
YAMAGUCHI, K
中科院分区：
文献类型：
--
作者：
HOSOKAWA, Y;MATSUMOTO, A;YAMAGUCHI, K
Cysteine dioxygenase is a key enzyme of cysteine metabolism with mammals. The cDNA clones for rat liver cysteine dioxygenase were isolated by immunological screening and plaque hybridization from a rat liver cDNA library. The longest clone contained an insert of the 1458 bp and encoded a polypeptide of 200 amino acids. The clone included the corresponding nucleotide sequence to amino acid sequences obtained from four lysyl endopeptidase-digested fragments of purified rat liver cysteine dioxygenase. The calculated molecular weight of rat liver cysteine dioxygenase was 23,025. Northern blot analysis revealed a single cysteine dioxygenase mRNA species of about 1.7 kb. A computer homology search indicated that protein showed no homology with any known protein.
