Imaging tumor angiogenesis with contrast ultrasound and microbubbles targeted to αvβ3
Imaging tumor angiogenesis with contrast ultrasound and microbubbles targeted to αvβ3
复制标题
DOI：
10.1161/01.cir.0000080326.15367.0c
复制
发表时间：
2003-07-22
期刊：
影响因子：
37.8
通讯作者：
Lindner, JR
中科院分区：
文献类型：
--
作者：
Ellegala, DB;Poi, HL;Lindner, JR
背景-血管生成是肿瘤生长和转移的关键决定因素。我们假设，造影增强超声（CEU）与微泡靶向α（V）-整合素表达的新生血管endothelial.Methods和Results-Malignant胶质瘤的新生血管endothelial.Methods和Results-Malignant胶质瘤在14只无胸腺大鼠脑内植入U87 MG人胶质瘤细胞。在植入后第14天或第28天，通过表面偶联echistatin，用靶向α（v）β（3）的微泡进行CEU。使用非靶向微泡CEU灌注成像获得肿瘤微血管血容量和血流速度。血管α（V）-整合素表达通过免疫组织化学进行评估，微泡粘附通过共聚焦显微镜进行表征。从14天到28天，平均肿瘤大小显著增加（2+/-1对35+/-14 mm（2），P
Background-Angiogenesis is a critical determinant of tumor growth and metastasis. We hypothesized that contrast-enhanced ultrasound (CEU) with microbubbles targeted to alpha(v)-integrins expressed on the neovascular endothelium could be used to image angiogenesis.Methods and Results-Malignant gliomas were produced in 14 athymic rats by intracerebral implantation of U87MG human glioma cells. On day 14 or day 28 after implantation, CEU was performed with microbubbles targeted to alpha(v)beta(3) by surface conjugation of echistatin. CEU perfusion imaging with nontargeted microbubbles was used to derive tumor microvascular blood volume and blood velocity. Vascular alpha(v)-integrin expression was assessed by immunohistochemistry, and microbubble adhesion was characterized by confocal microscopy. Mean tumor size increased markedly from 14 to 28 days (2+/-1 versus 35+/-14 mm(2), P