Influence of Ethylene on Morphology and Pigment Changes in Harvested Broccoli
Influence of Ethylene on Morphology and Pigment Changes in Harvested Broccoli
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DOI：
10.1007/s11947-019-02267-1
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发表时间：
2019-05-01
影响因子：
5.6
通讯作者：
Ji, Shu-juan
中科院分区：
文献类型：
--
作者：
Cai, Jia-hui;Cheng, Shun-chang;Ji, Shu-juan
采用乙烯和乙烯吸收塔处理，研究了乙烯含量对青花菜形态和色泽的影响。结果表明，未经处理的西兰花在10℃下采收8天后开始变黄，乙烯处理导致黄化提前2天发生，而乙烯吸收处理推迟并显著降低了黄化程度。黄化首先发生在花蕾的底部；当时，花蕾的形状没有明显变化，小花也没有开花。扫描电子显微镜显示，乙烯含量的增加导致花蕾细胞收缩，细胞排列紊乱。乙烯处理增加了叶绿素b(Chlide B)、脱镁叶绿酸B(Pheide B)、β-隐黄质、叶黄素和β-胡萝卜素的含量。黄化程度的增加导致类胡萝卜素而不是叶绿素成为关键色素(β-隐黄素、β-胡萝卜素和叶黄素与h度极显著相关)。在这一变化中，BoChl2、BoPaO、BoRCCR、BoPSY和BoLCYB基因的表达水平受到乙烯的影响较大。乙烯吸收处理并没有降低基因的表达，而是延缓了基因的表达。这些结果表明，乙烯吸收塔只吸收了产生的乙烯，并不直接作用于叶绿素的降解。乙烯还刺激BoCCD1和BoCCD7的表达，导致一种未知的黄色物质的产生，这种物质增加了西兰花的黄色。相比之下，BoCCD7的表达比BoCCD1更敏感。
Ethylene and ethylene absorber treatments were used to study the effects of ethylene content on the morphology and pigmentation of broccoli. Results showed that untreated broccoli began to turn yellow 8 days after being harvested at 10 degrees C. Ethylene treatment caused yellowing to occur 2 days earlier, and ethylene absorber treatment delayed and significantly reduced the degree of yellowing. Yellowing first occurred in the base of the bud; at that time, the shape of the bud had not changed significantly and there was no blooming of the florets. Scanning electron microscopy revealed that the increase in ethylene content caused the calyx cells of broccoli to shrink and that the cell arrangement had become disordered. Ethylene treatment caused increases in the content of chlorophyllide b (Chlide b), pheophorbide b (Pheide b), beta-cryptoxanthin, lutein, and beta-carotene. The increased degree of yellowing resulted in carotenoids rather than chlorophyll becoming the key pigment (beta-cryptoxanthin, beta-carotene, and lutein were very significantly correlated with h degrees). During this change, the expression levels of the BoChl2, BoPaO, BoRCCR, BoPSY, and BoLCYB genes were affected to a greater extent by ethylene. Ethylene absorber treatment did not reduce gene expression, but delayed it. These results indicate that the ethylene absorber only absorbed the ethylene produced and did not directly act on the degradation of chlorophyll. Ethylene also stimulated the expression of BoCCD1 and BoCCD7, resulting in the production of an unknown yellow substance that increased the yellow color of broccoli. In comparison, BoCCD7 expression was more sensitive than BoCCD1.