Chlorophyll fluorescence and gas exchange measurements in field research: an ecological case study
Chlorophyll fluorescence and gas exchange measurements in field research: an ecological case study
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DOI：
10.1007/s11099-018-0809-5
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发表时间：
2018-12-01
期刊：
影响因子：
2.7
通讯作者：
Roemermann, C.
中科院分区：
文献类型：
--
作者：
Bucher, S. F.;Bernhardt-Roemermann, M.;Roemermann, C.
我们测试了廉价和快速的叶绿素(Chl)荧光是否可以用于生态生理领域的研究，作为气体交换测量的替代品。测定了4种草本植物在饱和光照和大气CO2浓度下的净光合速率(P(Nsat))、最大羧化速率(V-CMAX)、PSII的最大量子效率(F-v/F-m)、性能指数(PIabs)、叶片氮(Narea)和碳同位素识别率(Delta C-13)。我们分析了Chl荧光与气体交换参数的关系，以及它们与通过生态生理性状间接评估植物表现的联系。F-v/F-m与P(Nsat)无相关性，与V-CMAX呈弱相关。PIabs与P(Nsat)、V-CMAX呈正相关。PIabs、P(Nsat)和V-Cmax与N面积呈正相关，与Delta C-13呈负相关，而F-v/F-m与N面积无关，与Delta C-13呈正相关。因此，当针对大量样品时，PIabs可能适用于表征光合作用活性。
We tested whether cheap and quick chlorophyll (Chl) fluorescence can be used in ecophysiological field studies as proxies for gas-exchange measurements. We measured net photosynthetic rate at saturating irradiance and ambient atmospheric CO2 concentrations (P (Nsat)), maximum carboxylation rate (V-cmax), maximum quantum yield of PSII (F-v/F-m), the performance index (PIabs), leaf nitrogen (Narea), and carbon isotope discrimination (Delta C-13) within four herbaceous species along two elevational gradients. We analysed the relationship between Chl fluorescence and gas-exchange parameters and their link to indirect assessment of plant performance via ecophysiological traits. F-v/F-m showed no relationship to P (Nsat) and only weak relationships to V-cmax. PIabs was positively related to P (Nsat) and V-cmax. PIabs, P (Nsat), and V-cmax were positively associated with N-area and negatively to Delta C-13, whereas F-v/F-m showed no relationship to Narea and a positive to Delta C-13. Thus, PIabs might be suitable to characterize the photosynthetic activity when aiming on large numbers of samples.