The Molecular Receptive Ranges of Human TAS2R Bitter Taste Receptors
The Molecular Receptive Ranges of Human TAS2R Bitter Taste Receptors
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DOI：
10.1093/chemse/bjp092
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发表时间：
2010-02-01
期刊：
影响因子：
3.5
通讯作者：
Behrens, Maik
中科院分区：
文献类型：
--
作者：
Meyerhof, Wolfgang;Batram, Claudia;Behrens, Maik
人类认为成千上万的化合物是苦的。与此形成鲜明对比的是，只有类似于25味觉2受体（TAS 2 R）的苦味受体已被确定，提出了一个问题，如何通过这样有限数量的传感器检测到大量的苦味化合物。为了解决这个问题，我们挑战了25人类味觉2受体（hTAS 2 R）与104天然或合成的苦味化学物质在异源表达系统。发现了5种孤儿受体的13种同源苦味化合物和64种先前鉴定的受体的新化合物。而一些受体只认识到少数激动剂，其他显示中度或极端调谐宽度。因此，3个hTAS 2 R一起能够检测到类似于50%的所用物质。相反，尽管63种苦味物质仅激活1-3种受体，但19种化合物刺激了多达15种hTAS 2 R。我们的数据表明，许多苦味化学物质的检测与hTAS 2 Rs的分子接受范围有关。
Humans perceive thousands of compounds as bitter. In sharp contrast, only similar to 25 taste 2 receptors (TAS2R) bitter taste receptors have been identified, raising the question as to how the vast array of bitter compounds can be detected by such a limited number of sensors. To address this issue, we have challenged 25 human taste 2 receptors (hTAS2Rs) with 104 natural or synthetic bitter chemicals in a heterologous expression system. Thirteen cognate bitter compounds for 5 orphan receptors and 64 new compounds for previously identified receptors were discovered. Whereas some receptors recognized only few agonists, others displayed moderate or extreme tuning broadness. Thus, 3 hTAS2Rs together were able to detect similar to 50% of the substances used. Conversely, though 63 bitter substances activated only 1-3 receptors, 19 compounds stimulated up to 15 hTAS2Rs. Our data suggest that the detection of the numerous bitter chemicals is related to the molecular receptive ranges of hTAS2Rs.