Microarray analysis of endometrial carcinomas and mixed mullerian tumors reveals distinct gene expression profiles associated with different histologic types of uterine cancer
Microarray analysis of endometrial carcinomas and mixed mullerian tumors reveals distinct gene expression profiles associated with different histologic types of uterine cancer
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DOI：
10.1158/1078-0432.ccr-04-2001
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发表时间：
2005-06-01
影响因子：
11.5
通讯作者：
Risinger, JI
中科院分区：
文献类型：
--
作者：
Maxwell, GL;Chandramouli, GVR;Risinger, JI
以往的研究表明，使用cDNA微阵列不同的基因表达谱的特点子宫内膜类浆液性癌和乳头状浆液性癌。分子生物学研究发现，混合苗勒管肿瘤，其特征在于两个癌性和肉瘤成分，共享的功能是子宫内膜癌的特征。本分析的目的是更精确地确定基因表达模式，区分子宫内膜癌和混合苗勒管肿瘤的浆液性和乳头状浆液性组织学。研究了119例病理学证实的子宫癌样本（66例子宫内膜样癌，24例乳头状浆液性肿瘤和29例混合性苗勒管肿瘤）。使用Affyscore人类基因组阵列U133A和U133B基因芯片组分析基因表达。非监督分析揭示了不同的全球基因表达模式的类胶质瘤，乳头状浆液性，混合苗勒氏管肿瘤，和正常组织作为严重分离的集群。两样本t检验比较类浆液性乳头状瘤、类浆液性苗勒管瘤和混合性苗勒管瘤以及乳头状浆液性苗勒管瘤和混合性苗勒管瘤对，分别鉴定出1，055、5，212和1，208个差异表达基因，P < 0.001。这些数据表明，不同的基因表达模式的特点不同的组织学类型的子宫癌。使用定量PCR确认选择基因的基因表达谱。了解各种组织学类型的子宫内膜癌的分子异质性有可能在未来实现更好的个性化治疗。
Previous studies using cDNA microarray have indicated that distinct gene expression profiles characterize endometrioid and papillary serous carcinomas of the endometrium. Molecular studies have observed that mixed mullerian tumors, characterized by both carcinomatous and sarcomatous components, share features that are characteristic of endometrial carcinomas. The objective of this analysis was to more precisely define gene expression patterns that distinguish endometrioid and papillary serous histologies of endometrial carcinoma and mixed mullerian tumors of the uterus. One hundred nineteen pathologically confirmed uterine cancer samples were studied (66 endometrioid, 24 papillary serous, and 29 mixed mullerian tumors). Gene expressions were analyzed using the Affymetrix Human Genome Arrays U133A and U133B Genechip set. Unsupervised analysis revealed distinct global gene expression patterns of endometrioid, papillary serous, mixed mullerian tumors, and normal tissues as grossly separated clusters. Two-sample t tests comparing endometrioid and papillary serous, endometrioid and mixed mullerian tumor, and papillary serous and mixed mullerian tumor pairs identified 1,055, 5,212, and 1,208 differentially expressed genes at P < 0.001, respectively. These data revealed that distinct patterns of gene expression characterize various histologic types of uterine cancer. Gene expression profiles for select genes were confirmed using quantitative PCR. An understanding of the molecular heterogeneity of various histologic types of endometrial cancer has the potential to lead to better individualization of treatment in the future.