Tissue-specific expression of the heat shock protein HSP27 during Drosophila melanogaster development.
Tissue-specific expression of the heat shock protein HSP27 during Drosophila melanogaster development.
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DOI：
10.1083/jcb.111.3.817
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发表时间：
1990-09
期刊：
影响因子：
--
通讯作者：
Arrigo AP
中科院分区：
文献类型：
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作者：
Pauli D;Tonka CH;Tissieres A;Arrigo AP
在黑腹果蝇发育过程中，α -结晶蛋白相关热休克（应激）蛋白hsp27在没有热休克的情况下表达。在这里，我们用免疫亲和纯化抗体描述了这种蛋白的组织分布。在胚胎中，由母体RNA翻译而来的热休克蛋白27除在卵黄中外分布均匀。在第一，第二和早期第三幼虫阶段，hsp27的表达仅限于大脑和性腺。这些组织的特点是高水平的增殖细胞。在三龄后期和早期蛹中，除中枢神经系统和性腺外，所有想像盘均合成hsp27。椎间盘的表达似乎仅限于分化的开始，因为它在蛹期的后半期消失了：在椎间盘衍生的成体器官中没有观察到更多的hsp27。在成人中，hsp27仍然存在于中枢神经系统的某些区域，也在男性和女性生殖系中表达，并在成熟精子和卵母细胞中积累。转录本和蛋白质自卵黄形成开始就在卵母细胞中积累，分布均匀，与胚胎中的分布相似。成年种系转录hsp27基因，而在蛹晚期和成年脑中未检测到转录物。这些结果提示hsp27在果蝇的发育过程中具有多种作用，可能与组织的增殖和分化状态有关。
The alpha-crystallin-related heat shock (stress) protein hsp27 is expressed in absence of heat shock during Drosophila melanogaster development. Here, we describe the tissue distribution of this protein using an immunoaffinity-purified antibody. In embryos, hsp27 translated from maternal RNA is uniformly distributed, except in the yolk. During the first, second, and early third larval stages, hsp27 expression is restricted to the brain and the gonads. These tissues are characterized by a high level of proliferating cells. In late third instar larvae and early pupae, in addition to the central nervous system and the gonads, all the imaginal discs synthesize hsp27. The disc expression seems restricted to the beginning of their differentiation since it disappears during the second half of the pupal stage: no more hsp27 is observed in the disc-derived adult organs. In adults, hsp27 is still present in some regions of the central nervous system, and is also expressed in the male and female germ lines where it accumulates in mature sperm and oocytes. The transcript and the protein accumulate in oocytes since the onset of vitellogenesis with a uniform distribution similar to that found in embryos. The adult germ lines transcribe hsp27 gene while no transcript is detected in the late pupal and adult brain. These results suggest multiple roles of hsp27 during Drosophila development which may be related to both the proliferative and differentiated states of the tissues.