PbGLR3.3 Regulates Pollen Tube Growth in the Mediation of Ca2+ Influx in Pyrus bretschneideri
PbGLR3.3 Regulates Pollen Tube Growth in the Mediation of Ca2+ Influx in Pyrus bretschneideri
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PbGLR3.3 通过介导梨树 Ca2+ 内流调节花粉管生长
DOI：
10.1007/s12374-016-0210-5
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发表时间：
2018-08-01
影响因子：
2.9
通讯作者：
Wu, Juyou
中科院分区：
文献类型：
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作者：
Fabrice, Musana Rwalinda;Jing, Yinghui;Wu, Juyou
谷氨酸受体 (GluR) 是细胞外信号的传感器；它们在真核生物多种生理和发育过程的调节中发挥着重要作用。然而，它们在果树中的功能作用在很大程度上尚不清楚。在此，基于本实验室建立的梨基因组数据库，我们在梨（Pyrus bretschneideri Rehd）中鉴定出34个PbGLR，并通过系统发育分析将其分为4类。与其他组相比，第 3 组中 PbGLR 的系统发育分析和结构信息表明这些基因具有特定特征。在第3组的10个基因中，我们观察到PbGLR3.3的表达在花粉萌发和持续生长过程中逐渐增加，表明该基因可能在梨花粉管的发育中发挥重要作用。利用反义寡脱氧核苷酸和Ca2+敏感荧光探针方法相结合，我们验证了PbGLR3.3参与DSer引发的细胞内Ca2+信号传导和梨花粉管生长的Ca2+调节。
Glutamate receptors (GluRs) are sensors of extracellular signals; they play important roles in the regulation of multiple physiological and developmental processes in eukaryotes. However, their functional roles in fruit trees are largely unknown. Here, based on the pear genome database, which was established in this lab, we identified 34 PbGLRs in pear (Pyrus bretschneideri Rehd), and they were divided into four groups by phylogenetic analysis. In comparisons with other groups, phylogenetic analyses and structural information of the PbGLRs in group 3 suggest that these genes underlie specific characteristics. Among the ten genes in group 3, we observed that the expression of PbGLR3.3 increased gradually during pollen germination and continuous growth, indicating that this gene might play a vital role in the development of pear pollen tubes. Using a combination of antisense oligodeoxy nucleotides and Ca2+-sensitive fluorescent probe methods, we verified that PbGLR3.3 participates in DSer-elicited intracellular Ca2+ signaling and Ca2+ regulation of growth in pear pollen tubes.