Connexin expression in homotypic and heterotypic cell coupling in the developing cerebral cortex
Connexin expression in homotypic and heterotypic cell coupling in the developing cerebral cortex
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DOI：
10.1002/cne.2121
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发表时间：
2002-02-11
影响因子：
2.5
通讯作者：
Parnavelas, JG
中科院分区：
文献类型：
--
作者：
Bittman, K;Becker, DL;Parnavelas, JG
通过缝隙连接通道进行细胞间通讯是大脑皮层发育的一个显著特征。在出生后的头两周，这是大鼠新皮质发育的关键时间，广泛的细胞耦合已被证明随着皮质的成熟而减弱。在缝隙连接蛋白家族中，连接蛋白26、36和43在皮质发育过程中差异表达。我们使用细胞内染料注射和连接蛋白免疫组织化学方法：研究了不同类型的神经细胞和神经胶质细胞在大鼠发育皮质中的偶联模式和连接蛋白的表达。我们发现，在出生后7天和14天，神经元和胶质细胞发生同型和异型耦合。虽然这种耦合的发生率是同型的，但也有相当多的异型耦合，涉及锥体和非锥体神经元，皮质的主要神经细胞类型，或神经元和星形胶质细胞。不同细胞类型之间的偶联似乎是通过连接蛋白26、36和43的差异表达来调节的。这可能是发育中的新皮质中的细胞之间的耦合是这些和其他连接蛋白在空间和时间表达的函数。J.Comp.神经。443：201-212,2002。(C)2002年Wiley-Liss，Inc.
Intercellular communication through gap junction channels is a prominent feature of the developing cerebral cortex. In the first 2 weeks after birth, a time critical in the development of the rat neocortex, extensive cell coupling has been documented that diminishes as the cortex matures. Among the family of gap junction proteins, connexins 26, 36, and 43 are differentially expressed during cortical development. We used intracellular dye injections and connexin immunohistochemistry to: investigate the coupling patterns and connexin expression between the different neuronal and glial cell types of the developing cortex of the rat. We found that neurons and glia couple homotypically and heterotypically at postnatal days 7 and 14. Although the prevalence of coupling was homotypic, there was considerable heterotypic coupling that involved pyramidal and nonpyramidal neurons, the principal neuronal cell types of the cortex, or neurons and astrocytes. Coupling between different cell types appeared to be mediated by differential expression of connexins 26, 36, and 43. It may be that coupling between cells in the developing neocortex is a function of the spatial and temporal expression of these and other connexin proteins. J. Comp. Neurol. 443:201-212, 2002. (C) 2002 Wiley-Liss, Inc.