Disruption of Candida albicans IFF4 gene involves modifications of the cell electrical surface properties
Disruption of Candida albicans IFF4 gene involves modifications of the cell electrical surface properties
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DOI：
10.1016/j.colsurfb.2007.03.017
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发表时间：
2007-08-01
影响因子：
5.8
通讯作者：
Cottin, Jane
中科院分区：
文献类型：
--
作者：
Kempf, Marie;Apaire-Marchais, Veronique;Cottin, Jane
在过去的二十年中，念珠菌病的患病率显着增加，白色念珠菌现已成为医院感染的最重要原因之一，特别是在惰性表面（如导管或假体）定植后。在以前的报告中，我们证明了35个未鉴定的基因的过度表达，以响应C。白色念珠菌芽管塑料。因此，进行了生物信息学分析，寻找编码可能参与粘附的表面蛋白的基因。因此选择了19个基因，并进一步研究了其中之一CaIFF 4。该CaIFF 4基因的推导蛋白揭示了糖基磷脂酰肌醇（GPI）锚定位点以及N-末端信号肽的存在。CaIFF 4基因的两个等位基因均被破坏。用PCR方法对白色念珠菌亲本菌株BWP 17进行扩增。然后对CaIFF 4基因无效突变体的性质进行了研究，结果表明，与亲本菌株BWP 17相比，芽管对塑料的粘附性降低。此外，CaIFF 4无效突变体和亲本菌株BWP 17的芽管电泳迁移率进行了测定。然后用软颗粒分析理论对数据进行分析。结果指出，与BWP 17亲本菌株的芽管相比，CaIFF 4无效突变体的芽管的电泳迁移率不太重要。(C)2007 Elsevier B. V.保留所有权利。
During the past two decades, the prevalence of candidiasis has increased markedly and Candida albicans has now become one of the most important causes of nosocomial infections, especially after colonization of inert surfaces such as catheters or prostheses. In a previous report, we demonstrated the overexpression of 35 unidentified genes in response to adherence of C. albicans germ tubes to plastic. Therefore, a bioinformatic analysis was performed searching for genes encoding surface proteins potentially involved in adherence. Nineteen genes were thus selected, and one of them, CaIFF4, was further investigated. The deduced protein of this CaIFF4 gene revealed a glycosylphosphatidylinositol (GPI)-anchored site as well as the presence of a N-terminal signal peptide. Disruption of both alleles of CaIFF4 gene from C. albicans parent strain BWP17 was performed by PCR method. Then investigations of properties of null mutant for CaIFF4 gene showed a decrease of adherence of germ tubes to plastic in comparison to the parent strain BWP17. Besides, electrophoretic mobilities of germ tubes of CaIFF4 null mutant and of parental strain BWP17 were measured. Data were then analysed with soft particles analysis theory. Results point out a less important electrophoretic mobility of germ tubes of CaIFF4 null mutant in comparison to germ tubes of BWP17 parental strain. (C) 2007 Elsevier B.V. All rights reserved.