Isoprenaline-induced transcription of 4 beta-galactosyltransferase is inhibited by both cycloheximide and actinomycin D in rat parotid acinar cells.
Isoprenaline-induced transcription of 4 beta-galactosyltransferase is inhibited by both cycloheximide and actinomycin D in rat parotid acinar cells.
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在大鼠腮腺腺泡细胞中，异丙肾上腺素诱导的 4β-半乳糖基转移酶转录可被放线菌酮和放线菌素 D 抑制。
DOI：
10.1042/bj2490357
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Humphreys-Beher,MG
中科院分区：
文献类型：
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作者：
Humphreys-Beher,MG
高尔基体酶4 β-半乳糖基转移酶的cDNA克隆（EC 2.4.1.38）用于测定培养的大鼠腮腺腺泡细胞中的稳态mRNA含量。异丙肾上腺素，β-肾上腺素能受体激动剂，造成增加的稳态量的mRNA为4 β-半乳糖基转移酶在培养的腺泡细胞，以及在比活性的酶。4 β-半乳糖基转移酶特异性mRNA的量依赖于基因的转录，如通过将细胞与RNA聚合酶抑制剂放线菌素D一起孵育以及异丙肾上腺素处理的时间所确定的。4 β-半乳糖基转移酶基因的转录也需要额外的细胞因子的主动生物合成，因为在与蛋白质合成抑制剂放线菌酮共孵育时未观察到异丙肾上腺素诱导的mRNA量增加。
A cDNA clone for the Golgi enzyme 4 beta-galactosyltransferase (EC 2.4.1.38) was used to determine the steady-state mRNA content in cultured rat parotid acinar cells. Isoprenaline, a beta-adrenergic-receptor agonist, caused an increase in steady-state amounts of mRNA for 4 beta-galactosyltransferase in cultured acinar cells as well as in specific activity of the enzyme. The amount of 4 beta-galactosyltransferase-specific mRNA was dependent on transcription of the gene, as determined by incubation of cells with the RNA polymerase inhibitor actinomycin D, concomitant with the time of isoprenaline treatment. Transcription of the 4 beta-galactosyltransferase gene also required the active biosynthesis of additional cellular factors, since isoprenaline-induced increases in mRNA amounts were not observed on co-incubation with the protein-synthesis inhibitor cycloheximide.