High-Fat Diet Increases Bone Loss by Inducing Ferroptosis in Osteoblasts.
High-Fat Diet Increases Bone Loss by Inducing Ferroptosis in Osteoblasts.
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DOI：
10.1155/2022/9359429
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发表时间：
2022
影响因子：
4.3
通讯作者：
Yu, Bin
中科院分区：
文献类型：
--
作者：
Zhu, RunJiu;Wang, ZhaoFu;Xu, Yuan;Wan, HaoYang;Zhang, Xin;Song, MingRui;Yang, Hong;Chai, Yu;Yu, Bin
目前的研究表明，慢性高脂肪饮食摄入可导致成年人骨质流失;然而，高脂肪饮食影响个体骨质疏松症发展的机制尚不清楚。由于高脂饮食与铁凋亡密切相关，铁凋亡是否介导高脂饮食诱导的骨丢失是我们目前研究的重点。通过将小鼠分为高脂饮食组、高脂饮食+铁凋亡抑制剂组和正常食物组，高脂组中的小鼠被给予高脂饮食12周。高脂饮食+铁凋亡抑制剂组小鼠在高脂饮食开始时每天腹腔注射1 mg/kg Fer-1。在建模期结束时，对所有三组的骨组织进行显微CT扫描、组织学检测和铁下垂的生化指标。将Mc 3 t3-E1细胞分为高脂培养液组、高脂培养液+铁凋亡抑制剂组和对照组。在高脂培养基中孵育24小时后，测定Mc 3 t3-E1细胞的铁凋亡标志物蛋白和生化参数，同时进行成骨诱导。在成骨诱导第7天测定细胞碱性磷酸酶含量和成骨相关蛋白的表达。结果表明，高脂饮食导致小鼠股骨骨丢失的发展，这一过程可以被铁凋亡抑制剂抑制。高脂饮食主要影响骨髓腔内生成的成骨细胞数量。体外高脂环境抑制成骨细胞增殖和成骨分化，并观察到铁中毒相关生化参数的显著变化。这些发现对未来高脂饮食引起的骨丢失的临床治疗具有重要意义。
Current research suggests that chronic high-fat dietary intake can lead to bone loss in adults; however, the mechanism by which high-fat diets affect the development of osteoporosis in individuals is unclear. As high-fat diets are strongly associated with ferroptosis, whether ferroptosis mediates high-fat diet-induced bone loss was the focus of our current study. By dividing the mice into a high-fat diet group, a high-fat diet + ferroptosis inhibitor group and a normal chow group, mice in the high-fat group were given a high-fat diet for 12 weeks. The mice in the high-fat diet + ferroptosis inhibitor group were given 1 mg/kg Fer-1 per day intraperitoneally at the start of the high-fat diet. Microscopic CT scans, histological tests, and biochemical indicators of ferroptosis were performed on bone tissue from all three groups at the end of the modelling period. Mc3t3-E1 cells were also used in vitro and divided into three groups: high-fat medium group, high-fat medium+ferroptosis inhibitor group, and control group. After 24 hours of incubation in high-fat medium, Mc3t3-E1 cells were assayed for ferroptosis marker proteins and biochemical parameters, and osteogenesis induction was performed simultaneously. Cellular alkaline phosphatase content and expression of osteogenesis-related proteins were measured at day 7 of osteogenesis induction. The results showed that a high-fat diet led to the development of femoral bone loss in mice and that this process could be inhibited by ferroptosis inhibitors. The high-fat diet mainly affected the number of osteoblasts produced in the bone marrow cavity. The high-fat environment in vitro inhibited osteoblast proliferation and osteogenic differentiation, and significant changes in ferroptosis-related biochemical parameters were observed. These findings have implications for the future clinical treatment of bone loss caused by high-fat diets.
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影响因子：
64.5
作者：
Dixon SJ;Lemberg KM;Lamprecht MR;Skouta R;Zaitsev EM;Gleason CE;Patel DN;Bauer AJ;Cantley AM;Yang WS;Morrison B 3rd;Stockwell BR
通讯作者：
Stockwell BR
影响因子：
3.7
作者：
Camirand A;Goltzman D;Gupta A;Kaouass M;Panda D;Karaplis A
通讯作者：
Karaplis A
血色素沉着症小鼠模型中铁死亡的特征
DOI：
10.1002/hep.29117
发表时间：
2017-08
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Wang H;An P;Xie E;Wu Q;Fang X;Gao H;Zhang Z;Li Y;Wang X;Zhang J;Li G;Yang L;Liu W;Min J;Wang F
通讯作者：
Wang F
影响因子：
5.8
作者：
Green JL;Dos Santos WF;Fontana ACK
通讯作者：
Fontana ACK
影响因子：
9.8
作者：
Pei, Zhaohui;Liu, Yandong;Ren, Jun
通讯作者：
Ren, Jun