Expression of the Celsr/flamingo homologue, c-fmi1, in the early avian embryo indicates a conserved role in neural tube closure and additional roles in asymmetry and somitogenesis
Expression of the Celsr/flamingo homologue, c-fmi1, in the early avian embryo indicates a conserved role in neural tube closure and additional roles in asymmetry and somitogenesis
复制标题
DOI：
10.1002/dvdy.20228
复制
发表时间：
2005-02-01
影响因子：
2.5
通讯作者：
Mason, I
中科院分区：
文献类型：
--
作者：
Formstone, CJ;Mason, I
火烈鸟是调节果蝇胚胎上皮结构平面细胞极性的核心蛋白群之一，而它们的脊椎动物对应蛋白在原肠胚形成和神经管闭合过程中涉及收敛伸展运动的协调，这表明平面极性机制也在这些过程中起作用。神经管关闭失败是人类最常见的出生缺陷之一，在两个表现神经管关闭失败的小鼠突变体中，发现了一个小鼠火烈鸟（fmi）同源基因Celsr1/fmi-1。这一失败导致脑后骨折，神经管从中脑后部打开。鸟类胚胎为研究神经管闭合提供了一个可操作的系统。我们已经鉴定了小鸡Celsr1/fmi-1同源基因c- fmi-1，并首次研究了鸟类火烈鸟基因的表达。我们发现，c-fmi1在早期胚胎中的表达是高度动态的，并且在神经管关闭开始时，c-fmi1转录本在鸟类神经上皮内变得丰富，这表明火烈鸟蛋白在这一过程中具有保守功能。我们的数据还表明c-fmi1在肌组发育中的作用。(C) 2004 Wiley-Liss, Inc。
Flamingo is one of a core group of proteins that regulate planar cell polarity of epithelial structures within the Drosophila embryo while their vertebrate counterparts have been implicated in the coordination of convergent extension movements during gastrulation and in neural tube closure, suggesting that planar polarity mechanisms also function in these processes. Failure of neural tube closure is one of the most common human birth defects, and a murine flamingo (fmi) homologue, Celsr1/fmi-1, was identified as the defective gene in two mouse mutants exhibiting failure of closure 1 of the neural tube. This failure resulted in craniorachischisis in which the neural tube is open from the midbrain posteriorly. The avian embryo provides a tractable system to study neural tube closure. We have identified a chick Celsr1/fmi-1 orthologue, c-fmi1 and provide the first study of expression of an avian flamingo gene. We show that expression is highly dynamic in the early embryo and that c-fmi1 transcripts become enriched within the avian neural epithelium at the initiation of neural tube closure, suggesting a conserved function for Flamingo proteins in this process. Our data also suggest a role for c-fmi1 in myotome development. (C) 2004 Wiley-Liss, Inc.