n-Electrode three-dimensional liquid chromatography with electrochemical detection for determination of neurotransmitters.
n-Electrode three-dimensional liquid chromatography with electrochemical detection for determination of neurotransmitters.
复制标题
正电极三维液相色谱与电化学检测用于测定神经递质。
作者：
W. Matson;P. Langlais;L. Volicer;P. Gamache;E. Bird;K. A. Mark
串联库仑电极已与液相色谱电化学检测一起使用，以增加组织神经递质直接分析的选择性和分辨率。电化学修饰、选择性和峰识别的三个库仑传感器的使用已经扩展到允许消除所有电化学不可逆物质的三个或四个库仑电极的“门”单元，以及用于通过其电流/电压特性分离共洗脱化合物的多达15个库仑电极的“阵列”单元。传感器阵列的柱上灵敏度为0.4至4 pg。门电池选择性有利于电化学可逆化合物而不是不可逆化合物，例如，3-甲氧基-4-羟基苯基乙二醇相对于抗坏血酸盐，高达10倍（4）。跨多电极阵列单元的分辨率允许分离具有相差小至30至40 mV的半波电位的共洗脱化合物。具有3至15个电极的细胞已用于测量脑中的单胺和代谢物;直接在血清滤液中的单胺;以及阿尔茨海默病患者脑脊液中5-羟色胺和5-羟色氨酸的氧化状态。
Coulometric electrodes in series have been used with liquid chromatography with electrochemical detection to increase selectivity and resolution for the direct analysis of tissue neurotransmitters. Use of three coulometric sensors for electrochemical modification, selectivity, and peak identification has been expanded into "gate" cells of three or four coulometric electrodes that allow elimination of all electrochemically irreversible substances, and "array" cells of up to 15 coulometric electrodes for separation of co-eluting compounds by their current/voltage characteristics. On-column sensitivity of the sensor arrays is 0.4 to 4 pg. Gate cell selectivity favors electrochemically reversible compounds over irreversible ones, e.g., 3-methoxy-4-hydroxyphenylglycol vs ascorbate, by a factor of up to 10(4). Resolution across the multi-electrode array cells allows separation of co-eluting compounds with half-wave potentials differing by as little as 30 to 40 mV. Cells with three to 15 electrodes have been used to measure monoamines and metabolites in brain; monoamines directly in serum filtrate; and the state of oxidation of 5-hydroxytryptamine and 5-hydroxytryptophan in cerebrospinal fluid of patients with Alzheimer's disease.