Transcription of vesicular stomatitis virus is required to shut off cellular RNA synthesis
Transcription of vesicular stomatitis virus is required to shut off cellular RNA synthesis
复制标题
水泡性口炎病毒的转录是关闭细胞 RNA 合成所必需的
作者：
P. Weck;R. Wagner
小鼠骨髓瘤（MPC-11）细胞的RNA合成迅速和逐步关闭水泡性口炎病毒温度敏感（ts）突变体允许转录感染。与此形成鲜明对比的是，突变体或缺陷性水疱性口炎病毒粒子限制在转录不能引起细胞RNA合成的进行性抑制，即使在大量的多重感染。tsG 114（I）在允许感染后30至60分钟合成的病毒产物似乎是在切换到限制性温度的细胞中延长RNA合成抑制所必需的。
RNA synthesis by mouse myeloma (MPC-11) cells was rapidly and progressively shut off by infection with vesicular stomatitis virus temperature-sensitive (ts) mutants permissive for transcription. In sharp contrast, mutants or defective vesicular stomatitis virions restricted in transcription were incapable of causing progressive inhibition of cellular RNA synthesis even at massive multiplicities of infection. A viral product synthesized 30 to 60 min after permissive infection with tsG114(I) appeared to be essential for prolonged inhibition of RNA synthesis in cells switched up to restrictive temperature.