THERMAL INACTIVATION OF CHERRY LEAF ROLL VIRUS IN TISSUE-CULTURES OF NICOTIANA-RUSTICA RAISED FROM SEEDS AND MERISTEM-TIPS
THERMAL INACTIVATION OF CHERRY LEAF ROLL VIRUS IN TISSUE-CULTURES OF NICOTIANA-RUSTICA RAISED FROM SEEDS AND MERISTEM-TIPS
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DOI：
10.1111/j.1744-7348.1978.tb00706.x
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发表时间：
1978-01-01
影响因子：
2.6
通讯作者：
WALKEY, DGA
中科院分区：
文献类型：
--
作者：
COOPER, VC;WALKEY, DGA
N.来源于用樱桃卷叶病毒（CLRV）感染的种子或胚的黄花菜组织培养物在22 ℃培养后保持感染。C.在32 ℃培养物中未发现感染性。但在将这种培养物转移到25 ℃后，很容易检测到。C为8天。在32 ℃温育20天后，病毒从大多数植物中被永久根除。在40 ℃孵育7天后，从所有植物中分离。C.部分纯化的CLRV制剂在22 ℃下9-12天后失去感染性。C，32 ℃下5天。在40 ℃下放置3天。C.
N. rustica tissue cultures derived from seeds or embryos infected with cherry leaf roll virus (CLRV), remained infected after culture at 22.degree. C. No infectivity was found in cultures held at 32.degree. C for 5 days but it was readily detected after such cultures were transferred to 25.degree. C for 8 days. Virus was permanently eradicated from most plants after 20 days incubation at 32.degree. C and from all plants after 7 days incubation at 40.degree. C. Partially purified preparations of CLRV lost infectivity after 9-12 days at 22.degree. C, 5 days at 32.degree. C and 3 days at 40.degree. C.