Molecular cloning and characterization of monoterpene synthases from Litsea cubeba (Lour.) Persoon
Molecular cloning and characterization of monoterpene synthases from Litsea cubeba (Lour.) Persoon
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DOI：
10.1007/s11295-011-0377-3
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发表时间：
2011-08-01
影响因子：
2.4
通讯作者：
Chu, Fang-Hua
中科院分区：
文献类型：
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作者：
Chang, Yueh-Te;Chu, Fang-Hua
山椒（Litsea cubeba）樟树（Persoon）是樟科常绿乔木，因其精油的抗氧化和抗菌作用而备受关注。毛茛挥发油的主要成分为单萜。为了研究长毛藤中具有生物活性的单萜类化合物的生物合成，分离了3个单萜类合成酶基因对应的cdna，并对其进行了体外功能表征。获得的基因分别命名为LcTPS1、LcTPS2和LcTPS3。LcTPS1的转录水平在叶组织中最为显著，LcTPS2和LcTPS3的转录水平在果组织中比在叶组织中更为明显。系统发育分析表明，LcTPS1、LcTPS2和LcTPS3均属于被子植物单萜合成酶TPS-b亚家族。对每个基因进行了相应酶的表达和功能表征。LcTPS1将二磷酸香叶基转化为反式辛烯，LcTPS2将二磷酸香叶基转化为α -thujene，而LcTPS3是一种多功能酶，分别将二磷酸香叶基转化为α -thujene和(+)-sabinene。
Mountain pepper (Litsea cubeba (Lour.) Persoon) is an evergreen tree in the Lauraceae family and has attracted attention for the antioxidant and antibacterial effects of its essential oils. The dominant components of the essential oil of L. cubeba are monoterpenes. To investigate the biosynthesis of monoterpenes that have bioactivity in L. cubeba, three cDNAs corresponding to monoterpene synthase genes were isolated, and the corresponding enzymes were functionally characterized in vitro. The genes obtained were named LcTPS1, LcTPS2, and LcTPS3. Transcript levels for LcTPS1 were most prominent in the leaf tissues, and levels of LcTPS2 and LcTPS3 were more evident in fruit tissues than in leaf tissues. Phylogenetic analyses indicated that LcTPS1, LcTPS2, and LcTPS3 all belong to TPS-b subfamily of angiosperm monoterpene synthases. For each genes, the corresponding enzymes were expressed and functionally characterized. LcTPS1 converted geranyl diphosphate to trans-ocimene, and LcTPS2 converted geranyl diphosphate to alpha-thujene, while LcTPS3 is a mutifunctional enzyme that converted geranyl diphosphate to alpha-thujene and (+)-sabinene, respectively.