Quantification of glycine betaine, choline and trimethylamine N-oxide in seawater particulates: Minimisation of seawater associated ion suppression
Quantification of glycine betaine, choline and trimethylamine N-oxide in seawater particulates: Minimisation of seawater associated ion suppression
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DOI：
10.1016/j.aca.2016.07.016
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发表时间：
2016-09-28
影响因子：
6.2
通讯作者：
Airs, Ruth
中科院分区：
文献类型：
--
作者：
Beale, Rachael;Airs, Ruth
建立了测定天然水样颗粒物中含氮渗透压(N-渗透压)的液-质联用(LC/MS)方法。充分的方法验证证明了该方法测定海水颗粒物中甘氨酸甜菜碱(GBT)、胆碱和三甲胺N-氧化物(TMAO)的有效性。GBT、胆碱和TMAO的检出限分别为3.5、1.2和5.9pg(相当于每升1.5、0.6和3.9nmoL)。该方法对GBT和胆碱的精密度通常为3%，对TMAO的精密度为6%。通过在线过滤实现了对天然样品的颗粒物分数的收集。在样品采集过程中，对玻璃纤维和聚碳酸酯过滤器的使用结果进行了分析和方法灵敏度评估和比较。离子抑制被证明是仪器对N-渗透压响应降低的一个重要原因，并与样品基质中海水的存在有关。(C)2016爱思唯尔B.V.保留所有权利。
A liquid chromatography/mass spectrometry (LC/MS, electrospray ionisation) method has been developed for the quantification of nitrogenous osmolytes (N-osmolytes) in the particulate fraction of natural water samples. Full method validation demonstrates the validity of the method for measuring glycine betaine (GBT), choline and trimethylamine N-oxide (TMAO) in particulates from seawater. Limits of detection were calculated as 3.5, 1.2 and 5.9 pg injected onto column (equivalent to 1.5, 0.6 and 3.9 nmol per litre) for GBT, choline and TMAO respectively. Precision of the method was typically 3% for both GBT and choline and 6% for TMAO. Collection of the particulate fraction of natural samples was achieved via in-line filtration. Resulting chromatography and method sensitivity was assessed and compared for the use of both glass fibre and polycarbonate filters during sample collection. Ion suppression was shown to be a significant cause of reduced instrument response to N-osmolytes and was associated with the presence of seawater in the sample matrix. (C) 2016 Elsevier B.V. All rights reserved.