Expression of midkine in normal and burn sites of rat skin
Expression of midkine in normal and burn sites of rat skin
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DOI：
10.1016/s0305-4179(98)00120-x
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发表时间：
1999-03-01
期刊：
影响因子：
2.7
通讯作者：
Muramatsu, T
中科院分区：
文献类型：
--
作者：
Iwashita, N;Muramatsu, H;Muramatsu, T
用免疫组化方法检测了大鼠正常皮肤和烧伤皮肤中中期因子（MK）的表达。在正常皮肤中，MK定位于表皮和真皮附属物如毛囊和皮脂腺。真皮下结缔组织中的肥大细胞也积累MK。烧伤后，MK阳性细胞开始向真皮下结缔组织浸润，伤后第2天达高峰，随后逐渐减少。正常和烧伤后皮肤的Western印迹分析显示，30 kDa的带与抗MK抗体反应，这条带的结论是MK的二聚体。本文从MK在创面愈合中的可能作用的角度对这些发现进行了讨论。(C)1999年由Elsevier Science Ltd和ISBI出版。All rights reserved.
Expression of midkine (MK), a retinoic acid-inducible heparin binding growth factor, was examined immunohistochemically in normal and burn sites of rat skin. In the normal skin, MK was localized in the epidermis and dermal appendages such as hair follicles and sebaceous glands. Mast cells in the subdermal connective tissue also accumulated MK. After burn injury, MK-positive cells began to infiltrate into subdermal connective tissue, and the number of MK-positive cells in the region increased to a maximum at postburn day two and then decreased gradually. Western blotting analysis of both normal and, postburn skin revealed a 30 kDa band reactive with anti-MK antibody; this band was concluded to be a dimer of MK. These findings were discussed from the viewpoint of the possible role of MK in wound healing. (C) 1999 Published by Elsevier Science Ltd and ISBI. All rights reserved.