The uptake and metabolism of fructose-1,6-diphosphate in rat cardiomyocytes
The uptake and metabolism of fructose-1,6-diphosphate in rat cardiomyocytes
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DOI：
10.1023/a:1010973806747
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发表时间：
2001-05-01
影响因子：
4.3
通讯作者：
Chien, S
中科院分区：
文献类型：
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作者：
Ehringer, WD;Chiang, B;Chien, S
果糖-1，6-二磷酸（FDP）是一种糖酵解中间体，理论上可增加缺血组织的代谢活性。在这里，我们研究了外部应用FDP对心肌细胞摄取和代谢的影响。分离成年大鼠心肌细胞，并暴露于不同浓度（0，5，2/5和50 mM）的FDP，缺氧（95% N-2/5% CO2）1，16或24小时，每个时间段，然后是1小时复氧（95%空气/5% CO2）。大鼠心肌细胞摄取FDP的浓度依赖性大于时间依赖性。此外，心肌细胞对FDP的摄取在缺氧和常氧处理的细胞中是相似的。Alamar Blue是一种对代谢活动敏感的氧化还原指示剂，用于监测FDP对心肌细胞代谢的影响。在1小时缺氧或常氧组，5，10和25 mM FDP显示出显着增加的代谢相比，对照细胞。当缺氧时间延长至16 h时，FDP各剂量组均高于对照组。在24 h缺氧或常氧时间段，只有10、25和50 mM FDP组大于对照组。结果表明，FDP的外部浓度和代谢中观察到的变化之间存在非线性趋势。这项研究的结果表明，5-10 mM之间的窄浓度范围增加心肌细胞代谢，但更高或更低的剂量可能几乎没有额外的影响。
Fructose-1,6-diphosphate (FDP) is a glycolytic intermediate which has been theorized to increase the metabolic activity of ischemic tissues. Here we examine the effects of externally applied FDP on cardiomyocyte uptake and metabolism. Adult rat cardiomyocytes were isolated and exposed to varying concentrations (0, 5, 25 and 50 mM) of FDP for either 1, 16 or 24 h of hypoxia (95% N-2/5% CO2), each time period followed by a 1 h reoxygenation (95% air/5% CO2). The uptake of FDP by rat cardiomyocytes was more concentration-dependent than time-dependent. Furthermore, the uptake of FDP by the cardiomyocytes was similar in the hypoxia and normoxia treated cells. Alamar Blue, a redox indicator that is sensitive to metabolic activity, was used to monitor the effects of the FDP on cardiomyocyte metabolism. In the 1 h hypoxia or normoxia group, the 5, 10 and 25 mM FDP showed a significant increase in metabolism compared to the control cells. When the length of hypoxia was extended to 16 h, all doses of FDP were greater than control. And at the 24 h hypoxia or normoxia time period, only the 10, 25 and 50 mM FDP groups were greater than control. The results indicate a non-linear trend between the external concentration of FDP and the changes noted in metabolism. The findings from this study indicate that a narrow concentration range between 5-10 mM augments cardiomyocyte metabolism, but higher or lower doses may have little additional affect.