Hydrogel microparticles for biosensing.
Hydrogel microparticles for biosensing.
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用于生物传感的水凝胶微粒。
DOI：
10.1016/j.eurpolymj.2015.02.022
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发表时间：
2015-11
影响因子：
6
通讯作者：
Doyle PS
中科院分区：
文献类型：
--
作者：
Le Goff GC;Srinivas RL;Hill WA;Doyle PS
由于其亲水性、生物相容性和高度可调性，近年来水凝胶材料在许多生物技术应用中引起了人们的强烈兴趣。特别是，它们在复杂生物样品中的溶液样环境和无污染性质使水凝胶成为生物传感应用的理想基质。水凝胶涂层和后来的凝胶点表面微阵列成功地用于灵敏的核酸测定和免疫测定。最近，用于从水凝胶材料合成编码颗粒的新的微制造技术已经使得能够开发基于水凝胶的悬浮阵列。光刻工艺和基于液滴的微流体技术使得能够生成具有独特光谱或图形代码的颗粒库，用于生物样品中的多路复用感测。在这篇综述中，我们讨论了设计水凝胶颗粒专用于生物传感时出现的关键问题。如何设计水凝胶材料以调整其特性并将生物探针置于其中？制造和编码凝胶颗粒的策略是什么？在检测后如何处理和解码颗粒？最后，我们回顾了迄今为止在文献中报道的使用水凝胶颗粒阵列的生物测定，并展望了该领域的进一步发展。
Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels as ideal substrates for biosensing applications. Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. More recently, new microfabrication techniques for synthesizing encoded particles from hydrogel materials have enabled the development of hydrogel-based suspension arrays. Lithography processes and droplet-based microfluidic techniques enable generation of libraries of particles with unique spectral or graphical codes, for multiplexed sensing in biological samples. In this review, we discuss the key questions arising when designing hydrogel particles dedicated to biosensing. How can the hydrogel material be engineered in order to tune its properties and immobilize bioprobes inside? What are the strategies to fabricate and encode gel particles, and how can particles be processed and decoded after the assay? Finally, we review the bioassays reported so far in the literature that have used hydrogel particle arrays and give an outlook of further developments of the field.