Localisation of glutathione and homoglutathione in Medicago truncatula is correlated to a differential expression of genes involved in their synthesis
Localisation of glutathione and homoglutathione in Medicago truncatula is correlated to a differential expression of genes involved in their synthesis
复制标题
DOI：
10.1046/j.1365-313x.1999.00367.x
复制
发表时间：
1999-01-01
期刊：
影响因子：
7.2
通讯作者：
Puppo, A
中科院分区：
文献类型：
--
作者：
Frendo, P;Gallesi, D;Puppo, A
对蒺藜苜蓿植株发育过程中的谷胱甘肽（GSH）和高谷胱甘肽（hGSH）进行了定量分析。hGSH仅在种子萌发后48小时可检测到，而GSH存在于干种子中，表明仅GSH用于种子中的硫储存。hGSH仅在成熟植株的地下部分可检测到，而GSH存在于所有器官中。γ-EC合成酶（γ-ECS）和GSH合成酶（GSH合成酶）在根和叶中有活性，而hGSH合成酶（hGSH合成酶）只在根中有活性。从M.蒺藜在γ-ECS缺陷型大肠杆菌的蛋白提取物中检测到高γ-ECS活性。coli菌株表达M.蒺藜北方杂交分析表明，γ-ECS基因在所有成熟的植物器官中表达相似，而gshs 1在叶和花中有较高的表达，而gshs 2在根和根瘤中优先表达。我们假设gshs 1和gshs 2分别编码GSHS和hGSHS。
Glutathione (GSH) and homoglutathione (hGSH) were quantified in Medicago truncatula during plant development. hGSH was detectable only 48 h after seed germination whereas GSH was present in the dry seeds, indicating that only GSH is used for sulphur storage in seeds. The hGSH was detectable only in the underground part of mature plants whereas GSH was present in all the organs. gamma-EC synthetase (gamma-ECS) and GSH synthetase (GSHS) activities were found in roots and leaves whereas hGSH synthetase (hGSHS) was found only in roots. Full-length cDNA encoding gamma-ECS and two partial cDNAs (gshs1 and gshs2) showing high identity with GSHS were isolated in M. truncatula. High gamma-ECS activity was detected in protein extracts of a gamma-ECS-deficient E. coli strain expressing the M. truncatula gamma-ECS. Northern blot analysis showed that the gamma-ECS gene was similarly expressed in all the mature plant organs tested, whereas gshs1 had a higher expression in leaves and flowers and gshs2 was preferentially expressed in roots and nodules. We hypothesise that gshs1 and gshs2 encode a GSHS and an hGSHS, respectively.