Distinctive KIR and HLA diversity in a panel of north Indian Hindus
Distinctive KIR and HLA diversity in a panel of north Indian Hindus
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DOI：
10.1007/s00251-001-0425-5
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发表时间：
2002-03-01
期刊：
影响因子：
3.2
通讯作者：
Parham, P
中科院分区：
文献类型：
--
作者：
Rajalingam, R;Krausa, P;Parham, P
HL A和KIR是由溶细胞淋巴细胞介导的多种快速进化的基因复合体，在人类免疫中共同发挥作用。要了解它们复杂的免疫遗传相互作用，需要研究同一人类群体中的人类白细胞抗原和KIR的多样性。在这里，一个由72名无关的北印度教徒组成的小组被分析了。通过基因组DNA测序或高分辨分型，确定HLA-A、B、C、DRB1、DQA1和DQB1等位基因及其频率；KIR基因分型通过KIR2DL1、2DL3、2DL5、3DL1和3DL2的基因特异性分型和等位基因水平的DNA分型确定。从人类白细胞抗原分析可以看出，北印度人与高加索人或东亚人有几个共同的特征，这与印度北部的人口学历史一致，也有一些特殊的特征，包括几个在其他人群中罕见或不存在的高频等位基因。大多数北印度人的KIR基因图谱在高加索人和亚洲人中都没有发现。最引人注目的是B组KIR单倍型频率较高。导致A组和B组单倍型在北印度人中的频率相等。北印度小组的72名成员都有不同的人类白细胞抗原基因和不同的KIR基因。
HLA and KIR are diverse and rapidly evolving gene complexes that work together in human immunity mediated by cytolytic lymphocytes. Understanding their complex immunogenetic interaction requires study of both HLA and KIR diversity in the same human population. Here a panel of 72 unrelated north Indian Hindus was analyzed. HLA-A, B, C, DRB1, DQA1, and DQB1 alleles and their frequencies were determined by sequencing or high-resolution typing of genomic DNA; KIR genotypes were determined by gene-specific typing and by allele-level DNA typing for KIR2DL1, 2DL3, 2DL5, 3DL1, and 3DL2. From HLA analysis, the north Indian population is seen to have several characteristics shared either with Caucasian or East Asian populations, consistent with the demographic history of north India, as well as specific features, including several alleles at high frequency that are rare or absent in other populations. A majority of the north Indian KIR gene profiles have not been seen in Caucasian and Asian populations. Most striking is a higher frequency of the B group of KIR haplotypes. resulting in equal frequencies for A and B group haplotypes in north Indians. All 72 members of the north Indian panel have different HLA genotype and different KIR genotype.