Effect of reduced dietary intake on energy expenditure, protein turnover, and glucose cycling in man.
Effect of reduced dietary intake on energy expenditure, protein turnover, and glucose cycling in man.
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DOI：
10.1016/0026-0495(91)90228-o
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发表时间：
1991-05
期刊：
影响因子：
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通讯作者：
T. Stein;W. Rumpler;M. Leskiw;M. Schluter;R. Staples;C. E. Bodwell
中科院分区：
文献类型：
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作者：
T. Stein;W. Rumpler;M. Leskiw;M. Schluter;R. Staples;C. E. Bodwell
在8名中度超重的男性中，研究了减少50%食物摄入量对能量消耗、蛋白质代谢、葡萄糖循环和身体成分的影响。研究前确定了8名受试者的平均卡路里和蛋白质摄入量。然后，他们在这种饮食中维持6周（平均±SEM， 3,269±75 kcal/d, 20.0±0.5 g N/d，第1期），之后在接下来的4周（1,555±38 kcal/d, 9.6±5 g N/d，第2期）中均匀减少主要食物的50%。在每个周期结束时，我们测量了(1)通过水下称重测量体脂和无脂质量，(2)在量热计中使用间接量热法测量24小时能量消耗，(3)15n甘氨酸的全身蛋白质合成和分解率，(4)葡萄糖和葡萄糖-6-磷酸之间的葡萄糖循环，果糖-6-磷酸和果糖-1,6二磷酸之间的果糖循环与6,6- d2和2- d1标记的葡萄糖。结果显示，在4周的减能方案中，受试者减脂4.0±0.1 kg（通过水下称重）。蛋白质周转和葡萄糖循环分别减少20%和15%。第1阶段24小时能量消耗为2553±166千卡/天，第2阶段为2369±69千卡/天，两者相差184±34千卡/天。综上所述，(1)虽然能量摄入减少了50%，但由于体脂的缓冲作用，能量消耗的减少幅度很小。(2)蛋白质周转和葡萄糖循环的减少约占两者之间184 kcal/d能量消耗差异的28%和7%。
The effect of a 50% reduction in food intake on energy expenditure, protein metabolism, glucose cycling, and body composition was investigated in eight moderately overweight men. The prestudy mean calorie and protein intake was determined for eight subjects. They were then maintained on this diet for 6 weeks (mean ± SEM, 3,269 ± 75 kcal/d, 20.0 ± 0.5 g N/d, period I), after which the diet was reduced uniformly in the major foodstuffs by 50% for the next 4 weeks (1,555 ± 38 kcal/d, 9.6 ± 5 g N/d, period II). At the end of each period we measured (1) body fat and fat free mass by underwater weighing, (2) 24-hour energy expenditure by indirect calorimetry in a calorimeter, (3) whole body protein synthesis and breakdown rates with15N glycine, and (4) glucose cycling between glucose and glucose-6-phosphate and fructose cycling between fructose-6-phosphate and fructose-1,6 bisphosphate with 6,6-D2- and 2-D1-labeled glucose. The results were subjects lost 4.0 ± 0.1 kg fat (by underwater weighing) during the 4 weeks on the reduced-energy regimen. Protein turnover and glucose cycling were reduced by 20% and 15%, respectively. Twenty-four-hour energy expenditure was 2,553 ± 166 kcal/d for period I and 2,369 ± 69 kcal/d for period II, giving a difference of 184 ± 34 kcal/d between the two periods. In conclusion, (1) although energy intake was reduced by 50%, the decrease in energy expenditure was small due to the buffering effect of body fat. (2) The reductions in protein turnover and glucose cycling could account for approximately 28% and 7% of the 184-kcal/d difference in energy expenditure between the two periods.