Artificial Cell Membrane-Covered Nanoparticles Embedding Quantum Dots as Stable and Highly Sensitive Fluorescence Bioimaging Probes
Artificial Cell Membrane-Covered Nanoparticles Embedding Quantum Dots as Stable and Highly Sensitive Fluorescence Bioimaging Probes
复制标题
DOI：
10.1021/bm800819r
复制
发表时间：
2008-11-01
影响因子：
6.2
通讯作者：
Ishihara, Kazuhiko
中科院分区：
文献类型：
--
作者：
Goto, Yusuke;Matsuno, Ryosuke;Ishihara, Kazuhiko
为了获得稳定且高灵敏度的生物成像荧光探针，用人工细胞膜覆盖嵌入有量子点的聚合物纳米颗粒。这些纳米颗粒通过组装磷脂极性基团作为平台，并将寡肽作为生物亲和部分固定在纳米颗粒的表面。由于磷酸胆碱基团的性质，聚合物纳米颗粒显示出对HeLa细胞的细胞摄取的抗性。当精氨酸八肽固定在纳米粒表面时，它们能够有效地穿透HeLa细胞膜。即使在固定寡肽后，也没有观察到纳米颗粒的细胞毒性。因此，我们获得了覆盖有人工细胞膜的稳定的荧光聚合物纳米颗粒，其可用作优异的生物成像探针和作为靶细胞中寡肽功能的新型评价工具。
To obtain a stable and highly sensitive bioimaging fluorescence probe, polymer nanoparticles with embedded quantum dots were covered with an artificial cell membrane. These nanoparticles were designed by assembling phospholipid polar groups as a platform, and oligopeptide was immobilized as a bioaffinity moiety on the surface of the nanoparticles. The polymer nanoparticles showed resistance to cellular uptake from HeLa cells owing to the nature of the phosphorylcholine groups. When arginine octapeptide was immobilized at the surface of the nanoparticles, they were able to penetrate the membrane of HeLa cells effectively. Cytotoxicty of the nanoparticles was not observed even after immobilization of oligopeptide. Thus, we obtained stable fluorescent polymer nanoparticles covered with an artificial cell membrane, which are useful as an excellent bioimaging probe and as a novel evaluation tool for oligopeptide functions in the target cells.