Amperometric biosensor based on multilayer containing carbon nanotube, plasma-polymerized film, electron transfer mediator phenothiazine, and glucose dehydrogenase
Amperometric biosensor based on multilayer containing carbon nanotube, plasma-polymerized film, electron transfer mediator phenothiazine, and glucose dehydrogenase
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DOI：
10.1016/j.bioelechem.2011.09.001
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发表时间：
2012-04-01
影响因子：
5
通讯作者：
Muguruma, Hitoshi
中科院分区：
文献类型：
--
作者：
Hoshino, Tatsuya;Sekiguchi, Shin-ichiro;Muguruma, Hitoshi
我们报告了一种新的电流型生物传感器的制作方法的基础上的多层膜含有碳纳米管（CNT），纳米薄等离子体聚合膜（PPF），电子转移介体吩噻嗪（PT），和酶葡萄糖脱氢酶（GDH）。电化学电极的结构依次由溅射金、乙腈PPF、PT、GDH和乙腈PPF（表示为PPF/GDH/PT/CNT/PPF/Au）组成。首先，沉积在Au上的PPF充当选择性渗透膜和CNT层形成的支架。第二PPF直接沉积在GDH上作为酶固定化的基质。为了促进CNT层和GDH之间的电化学连通，用氮等离子体处理CNT。电子转移介体PT起到将酶反应引起的电子转移到电极的介体的作用。介体和CNT之间的协同作用在降低操作潜力和增强灵敏度（电流）方面提供了好处。优化的葡萄糖生物传感器显示在+0.2 V vs. Ag/AgCl时的灵敏度为5.1 +/- 0.9 μ A mM（-1）cm（-2），线性动态范围为4.9-19 mM，响应时间为5 +/- 1 s。与与大规模生产技术不兼容的传统湿化学工艺不同，这种干化学工艺具有实现生物电子器件高通量生产的巨大潜力。此外，这些器件可以作为有用的、有用的或不可缺少的工具应用于细胞生物学功能领域并扩展。(C)2011 Elsevier B. V.保留所有权利。
We report on a novel fabrication approach of amperometric biosensor based on multilayer films containing carbon nanotubes (CNT), a nano-thin plasma-polymerized film (PPF), electron transfer mediator phenothiazine (PT), and enzyme glucose dehydrogenase (GDH). The configuration of the electrochemical electrode is sequentially composed of sputtered gold, acetonitrile PPF, PT, GDH, and acetonitrile PPF (denoted as PPF/GDH/PT/CNT/PPF/Au). First PPF deposited on Au acts as a permselective membrane and as a scaffold for CNT layer formation. Second PPF directly deposited on GDH acts as a matrix for enzyme immobilization. To facilitate the electrochemical communication between the CNT layer and GDH, CNT was treated with nitrogen plasma. The electron transfer mediator PT plays a role as the mediator in which the electron caused by enzymatic reaction transports to the electrode. The synergy between the mediator and CNT provides benefits in terms of lowering the operational potential and enhancing the sensitivity (current). The optimized glucose biosensor revealed a sensitivity of 5.1 +/- 0.9 mu A mM(-1) cm(-2) at +0.2 V vs. Ag/AgCl, linear dynamic range of 4.9-19 mM, and a response time of 5 +/- 1 s. Unlike conventional wet-chemical processes that are incompatible with mass production techniques, this dry-chemistry procedure has great potential for enabling high-throughput production of bioelectronic devices. Furthermore, those devices can be applied and expands for the cell biological functional field as a useful, helpful, or indispensable tool. (C) 2011 Elsevier B.V. All rights reserved.