Crumbs proteins stabilize the cone mosaics of photoreceptors and improve vision in zebrafish
Crumbs proteins stabilize the cone mosaics of photoreceptors and improve vision in zebrafish
复制标题
碎屑蛋白稳定光感受器的视锥细胞嵌合体并改善斑马鱼的视力
DOI：
10.1016/j.jgg.2020.12.002
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发表时间：
2021-01-20
影响因子：
5.9
通讯作者：
Zou, Jian
中科院分区：
文献类型：
--
作者：
Hao, Qinlong;Zheng, Mingjie;Zou, Jian
虽然脊椎动物锥嵌合体的独特组织在很久以前就被首次描述，但其潜在的分子基础和生理意义在很大程度上是未知的。在这里，我们表明，Crumbs蛋白，上皮顶端极性的关键调节，建立平面细胞的光感受器在斑马鱼的极性。通过异嗜性Crb 2a-Crb 2b相互作用，顶基极性蛋白Crb 2b限制Crb 2a在光感受器中的不对称平面分布。因此，平面极化的Crumbs蛋白平衡细胞间粘附和感光细胞之间的张力，从而稳定视锥嵌合体的几何组织。值得注意的是，斑马鱼中Crb 2b的缺失诱导斑马鱼的近视样表型，伴随着细长的眼轴，并损害斑马鱼对捕食的视觉感知。这些数据揭示了一个详细的机制，锥镶嵌稳态通过以前未发现的顶点平面极性协调，并提出了近视的致病机制。中国科学院遗传与发育生物学研究所版权所有（C）2021由Elsevier Limited和Science Press出版。All rights reserved.
Although the unique organization of vertebrate cone mosaics was first described long ago, both their underlying molecular basis and physiological significance are largely unknown. Here, we demonstrate that Crumbs proteins, the key regulators of epithelial apical polarity, establish the planar cellular polarity of photoreceptors in zebrafish. Via heterophilic Crb2a-Crb2b interactions, the apicobasal polarity protein Crb2b restricts the asymmetric planar distribution of Crb2a in photoreceptors. The planar polarized Crumbs proteins thus balance intercellular adhesions and tension between photoreceptors, thereby stabilizing the geometric organization of cone mosaics. Notably, loss of Crb2b in zebrafish induces a nearsightedness-like phenotype in zebrafish accompanied by an elongated eye axis and impairs zebrafish visual perception for predation. These data reveal a detailed mechanism for cone mosaic homeostasis via previously undiscovered apical-planar polarity coordination and propose a pathogenic mechanism for nearsightedness. Copyright (C) 2021, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.