Dopamine modifies oxygen consumption and mitochondrial membrane potential in striatal mitochondria
Dopamine modifies oxygen consumption and mitochondrial membrane potential in striatal mitochondria
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DOI：
10.1007/s11010-010-0456-z
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发表时间：
2010-08-01
影响因子：
4.3
通讯作者：
Lores-Arnaiz, Silvia
中科院分区：
文献类型：
--
作者：
Czerniczyniec, Analia;Bustamante, Juanita;Lores-Arnaiz, Silvia
多巴胺是一种与线粒体功能障碍有关的神经递质。在这项研究中，纹状体完整的线粒体和submitochondrial膜孵育不同的多巴胺浓度，线粒体功能，过氧化氢和一氧化氮的产生的变化进行了评估。在1 mM多巴胺孵育后，发现状态3氧摄取（主动呼吸状态）减少35%。此外，线粒体呼吸控制显著降低，表明线粒体功能障碍。高浓度多巴胺诱导线粒体去极化。此外，完整的纹状体线粒体的过氧化氢生产的评估显示0.5和1 mM多巴胺孵育后显着增加。孵育与0.5和1 mM多巴胺增加一氧化氮的生产在submitochondrial膜的28%和49%，分别与对照值相比。这项研究提供的证据表明，高多巴胺浓度诱导纹状体线粒体功能障碍，通过减少线粒体呼吸控制和膜电位的损失，可能介导的自由基的产生。
Dopamine is a neurotransmitter that has been related to mitochondrial dysfunction. In this study, striatal intact mitochondria and submitochondrial membranes were incubated with different dopamine concentrations, and changes on mitochondrial function, hydrogen peroxide, and nitric oxide production were evaluated. A 35% decrease in state 3 oxygen uptake (active respiration state) was found after 1 mM dopamine incubation. In addition, mitochondrial respiratory control significantly decreased, indicating mitochondrial dysfunction. High dopamine concentrations induced mitochondrial depolarization. Also, evaluation of hydrogen peroxide production by intact striatal mitochondria showed a significant increase after 0.5 and 1 mM dopamine incubation. Incubation with 0.5 and 1 mM dopamine increased nitric oxide production in submitochondrial membranes by 28 and 49%, respectively, as compared with control values. This study provides evidence that high dopamine concentrations induce striatal mitochondrial dysfunction through a decrease in mitochondrial respiratory control and loss of membrane potential, probably mediated by free radical production.