Bridging Rare and Abundant Bacteria with Ecosystem Multifunctionality in Salinized Agricultural Soils: from Community Diversity to Environmental Adaptation.
Bridging Rare and Abundant Bacteria with Ecosystem Multifunctionality in Salinized Agricultural Soils: from Community Diversity to Environmental Adaptation.
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DOI：
10.1128/msystems.01221-20
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发表时间：
2021-03-30
期刊：
影响因子：
6.4
通讯作者：
Huang Q
中科院分区：
文献类型：
--
作者：
Wan W;Liu S;Li X;Xing Y;Chen W;Huang Q
细菌多样性和生态系统多功能性（EMF）随环境梯度沿着变化。然而，很少有人知道EMF和稀有和丰富的细菌的分类和系统发育之间的相互联系。利用MiSeq测序和多重统计分析，我们评估了稀有和丰富的细菌的分类和系统发育差异的维持及其对盐碱化农业土壤中EMF的贡献（0.09至19.91 dS/m）。稀有细菌比丰富细菌具有更紧密的系统发育聚类和更广泛的环境宽度，而丰富细菌比稀有细菌具有更高的功能冗余和更强的系统发育信号的生态偏好。变异选择（86.7%）决定了稀有细菌群落的聚集，扩散限制（54.7%）和变异选择（24.5%）决定了丰富的细菌群落聚集。盐度在调节随机过程和确定性过程之间的平衡中起着决定性的作用，对稀有细菌和丰富细菌的功能和多样性都有显著影响。稀有细菌分类α多样性和丰富细菌系统发育α多样性对EMF的贡献显著，而丰富细菌分类α多样性和稀有细菌系统发育α多样性对EMF的贡献不显著。此外，丰富而不是罕见的细菌群落功能对土壤电动势有显着影响。这些发现扩展了我们对稀有和丰富细菌环境适应的认识，并强调了稀有和丰富细菌的分类和系统发育α多样性对土壤电动势的不同贡献。土壤盐渍化是一个世界性的环境问题，威胁着植物生产力和微生物多样性。了解微生物多样性的产生和维持对于通过调查生态系统多功能性来评估土壤耕作潜力至关重要。基于序列分析的结果表明，稀有细菌和丰富细菌在分类和系统发育水平上的环境适应性存在差异，从而导致稀有细菌和丰富细菌的分类和系统发育α-二聚体对土壤电动势的贡献不同。研究盐渍化土壤中稀有和丰富的细菌多样性及其对电动势的贡献对于指导土壤修复具有重要意义。
Bacterial diversity and ecosystem multifunctionality (EMF) vary along environmental gradients. However, little is known about interconnections between EMF and taxonomic and phylogenetic diversities of rare and abundant bacteria. Using MiSeq sequencing and multiple statistical analyses, we evaluated the maintenance of taxonomic and phylogenetic diversities of rare and abundant bacteria and their contributions to EMF in salinized agricultural soils (0.09 to 19.91 dS/m). Rare bacteria exhibited closer phylogenetic clustering and broader environmental breadths than abundant ones, while abundant bacteria showed higher functional redundancies and stronger phylogenetic signals of ecological preferences than rare ones. Variable selection (86.7%) dominated rare bacterial community assembly, and dispersal limitation (54.7%) and variable selection (24.5%) determined abundant bacterial community assembly. Salinity played a decisive role in mediating the balance between stochastic and deterministic processes and showed significant effects on functions and diversities of both rare and abundant bacteria. Rare bacterial taxonomic α-diversity and abundant bacterial phylogenetic α-diversity contributed significantly to EMF, while abundant bacterial taxonomic α-diversity and rare bacterial phylogenetic α-diversity did not. Additionally, abundant rather than rare bacterial community function had a significant effect on soil EMF. These findings extend our knowledge of environmental adaptation of rare and abundant bacteria and highlight different contributions of taxonomic and phylogenetic α-diversities of rare and abundant bacteria to soil EMF. IMPORTANCE Soil salinization is a worldwide environmental problem and threatens plant productivity and microbial diversity. Understanding the generation and maintenance of microbial diversity is essential to estimate soil tillage potential via investigating ecosystem multifunctionality. Our sequence-based data showed differences in environmental adaptations of rare and abundant bacteria at taxonomic and phylogenetic levels, which led to different contributions of taxonomic and phylogenetic α-diversities of rare and abundant bacteria to soil EMF. Studying the diversity of rare and abundant bacteria and their contributions to EMF in salinized soils is critical for guiding soil restoration.