Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level
Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level
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DOI：
10.1073/pnas.0907367106
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发表时间：
2009-10-20
影响因子：
11.1
通讯作者：
Alivisatos, A. Paul
中科院分区：
文献类型：
--
作者：
Jun, Young-Wook;Sheikholeslami, Sassan;Alivisatos, A. Paul
金属纳米颗粒中等离子体激元耦合的使用在许多生物过程的光学表征中显示出巨大的潜力。最近，我们已经证明了使用“等离子体规则”观察体外单个生物分子的构象变化。等离激元标尺提供稳健的信号而没有光漂白或闪烁。在这里，我们展示了第一个应用程序的等离子体统治者在体内研究观察活细胞中的单个生物分子的很长的轨迹。我们提出了一种独特类型的等离子体统治者组成的肽连接的金纳米粒子卫星周围的核心颗粒，这是用来作为探针，光学遵循细胞信号转导通路在体内的单分子水平。这些“冠状纳米颗粒等离子体规则”使我们能够连续监测活细胞中caspase-3活性的轨迹超过2小时，提供足够的时间来观察早期caspase-3活化，这是传统的整体分析所不可能的。
The use of plasmon coupling in metal nanoparticles has shown great potential for the optical characterization of many biological processes. Recently, we have demonstrated the use of "plasmon rulers'' to observe conformational changes of single biomolecules in vitro. Plasmon rulers provide robust signals without photo-bleaching or blinking. Here, we show the first application of plasmon rulers to in vivo studies to observe very long trajectories of single biomolecules in live cells. We present a unique type of plasmon ruler comprised of peptide-linked gold nanoparticle satellites around a core particle, which was used as a probe to optically follow cell-signaling pathways in vivo at the single-molecule level. These "crown nanoparticle plasmon rulers'' allowed us to continuously monitor trajectories of caspase-3 activity in live cells for over 2 h, providing sufficient time to observe early-stage caspase-3 activation, which was not possible by conventional ensemble analyses.