Colorimetric and chemiluminescence based enzyme linked apta-sorbent assay (ELASA) for ochratoxin A detection
Colorimetric and chemiluminescence based enzyme linked apta-sorbent assay (ELASA) for ochratoxin A detection
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DOI：
10.1016/j.saa.2020.118875
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发表时间：
2021-01-05
影响因子：
4.4
通讯作者：
Bhatt, Praveena
中科院分区：
文献类型：
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作者：
Mukherjee, Monali;Nandhini, C.;Bhatt, Praveena
赭曲霉毒素A(OTA)是一种分布最广的真菌毒素，可污染各种食品，如谷类、香料、花生、咖啡、葡萄酒、啤酒等，也可从受污染的饲料和饲料传播到食用它的动物的牛奶、血液、肉类、肾脏和肝脏。与生物识别分子相连的酶，如抗体或适配子，由于它们能够被用作生物传感格式的标签或标签而非常受欢迎。在这项工作中，评估了基于OTA适配子的比色和化学发光生物传感形式对OTA的检测。在优化的缓冲条件下，Co-ELASA和CL-ELASA的线性范围分别为1 pg/m L~1 mU g/m L和1.29 pg/m L，检出限分别为0.84pg/m L(定量限=2.54 pg/m L)和1.29 pg/m L(L=3.94 pg/m L)。ELASA方法与夹心ELISA法的比较表明，所建立的方法具有与常规方法相似的灵敏度，检出限为1.13pg/m L，检出限为3.41 pg/m L。通过在花生和咖啡豆中添加0.1、1和10 ppb的邻苯二甲酸二乙酯对模拟污染食品样品的研究表明，Co-ELASA的回收率为50.21%~113.27%，Cl-ELASA的回收率为90.47%~107.72%，ELISA的回收率为76.23%~141.49%。研究结果表明，Co-ELASA和CI-ELASA可作为食品样品中OTA超灵敏检测的替代方法，也可用于生物传感器的研制。(C)2020爱思唯尔B.V.保留所有权利。
Ochratoxin A (OTA) is one of the most widespread mycotoxin found to contaminate various food products such as cereals, spices, groundnuts, coffee, wine, beer etc. It is also carried over from contaminated feed and fodder to milk, blood, meat, kidney and liver of animals consuming it. Enzyme-linked to biorecognition molecules like antibodies or aptamers are very popular due to their ability to be used as labels or tags in biosensing formats. In this work, OTA aptamer based colorimetric and chemiluminescence biosensing formatswere evaluated for the detection of OTA. The colorimetric enzyme linked apta-sorbent assay (Co-ELASA) and chemiluminescence enzyme linked apta-sorbent assay (Cl-ELASA) showed a linear detection range from 1 pg/mL to 1 mu g/mL with a limit of detection (LOD) of 0.84 pg/mL for Co-ELASA (limit of quantification (LOQ) = 2.54 pg/mL) and 1.29 pg/mL for Cl-ELASA (LOQ= 3.94 pg/mL) under optimized buffer conditions. Comparison of ELASA methods with sandwich ELISA indicated that the developed techniques had sensitivity similar to the conventional technique which indicated a LOD of 1.13 pg/mL and LOQ of 3.41 pg/mL. Studies in simulated contaminated food samples by spiking OTA in groundnut and coffee bean at concentrations of 0.1, 1 and 10 ppb, indicated recoveries in the range of 50.21 to 113.27% for Co-ELASA, 90.47 to 107.72% for Cl-ELASA and 76.23 to 141.49% for ELISA. Results of the study indicate that Co-ELASA and Cl-ELASA assays could be an alternate approach for ultrasensitive detection of OTA in food samples, which can also be adapted for biosensor development. (C) 2020 Elsevier B.V. All rights reserved.