Involvement of Plasma Membrane Alterations in Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma).
Involvement of Plasma Membrane Alterations in Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma).
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DOI：
10.1104/pp.75.3.818
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发表时间：
1984-07
期刊：
影响因子：
7.4
通讯作者：
M. Uemura;S. Yoshida
中科院分区：
文献类型：
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作者：
M. Uemura;S. Yoshida
利用双水相分离技术从黑麦幼苗中分离出高纯度的质膜，测定了黑麦幼苗抗寒性形成过程中脂类和蛋白质的变化，其中脂肪酸不饱和度和磷脂的比例在冷驯化过程中变化不大。然而，在冷驯化过程中，观察到了甾醇成分的微小变化；β-谷甾醇逐渐增加，而菜籽甾醇和豆甾醇则相应减少。抗寒性越强，磷脂与蛋白质的比例越大。在驯化过程中，醇磷比下降，与抗寒性的发展呈负相关。在十二烷基硫酸钠平板聚丙烯酰胺凝胶上观察到未驯化和驯化的质膜多肽成分有明显的差异。糖肽中也检测到了变化。这些结果表明，在冷驯化过程中，质膜处于一种动态状态，并随着抗寒性的发展而变化。
Using plasma membranes with high purity isolated from winter rye seedlings (Secale cereale L. cv Puma) by an aqueous two-polymer phase partition technique, lipid and protein changes were determined during the development of cold hardiness.The degree of fatty acid unsaturation and proportions of phospholipid classes changed only slightly during cold acclimation. A small change, however, was observed in sterol composition; beta-sitosterol increased gradually with a corresponding decrease in campesterol plus stigmasterol during cold acclimation. The phospholipid to protein ratio increased proportionally to the increase in cold hardiness. During the period of acclimation, the sterol to phospholipid ratio declined and was inversely related to the development of cold hardiness.A distinct difference between polypeptide components of nonacclimated and acclimated plasma membrane was observed on two-dimensional sodium dodecyl sulfate slab polyacrylamide gels. A change was also detected in glycopeptides. These results suggest that the plasma membrane is in a dynamic state during cold acclimation, changing in response to the development of cold hardiness.