Taurine reduces caspase-8 and caspase-9 expression induced by ischemia in the mouse hypothalamic nuclei
Taurine reduces caspase-8 and caspase-9 expression induced by ischemia in the mouse hypothalamic nuclei
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DOI：
10.1007/s00726-006-0405-z
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发表时间：
2006
期刊：
影响因子：
3.5
通讯作者：
A. Taranukhin;Elena Y. Taranukhina;P. Saransaari;I. M. Djatchkova;M. Pelto-huikko;S. Oja
中科院分区：
文献类型：
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作者：
A. Taranukhin;Elena Y. Taranukhina;P. Saransaari;I. M. Djatchkova;M. Pelto-huikko;S. Oja
牛磺酸是一种在神经系统中含量丰富的含硫氨基酸。它可以保护细胞免受缺血诱导的细胞凋亡，但其背后的机制尚未明确。我们研究的目的是探讨牛磺酸对缺血引起的细胞凋亡的两个主要途径的影响：受体介导的细胞死亡和线粒体细胞死亡。含有下丘脑视上核 (SON) 和室旁核 (PVN) 的脑切片在不存在和存在 20 mM 牛磺酸的情况下，在控制和模拟缺血（缺氧-葡萄糖 30 分钟）条件下进行体外培养。 180 分钟“缺血后”期后收获脑切片并固定在 4% 多聚甲醛中。为了估计细胞凋亡，在石蜡包埋切片中对 caspase-8 和 caspase-9 进行免疫染色。所有实验组的SON和PVN中均观察到免疫反应性caspase-8和caspase-9细胞，但在“缺血”组中，两个下丘脑核团中caspase-8和caspase-9的表达以及免疫反应性细胞的数量均显着增加。与未经牛磺酸处理的“缺血”组相比，在 SON 和 PVN 缺血后，向培养介质中添加牛磺酸 (20 mM) 会导致 caspase-8 和 caspase-9 免疫反应性显着降低。牛磺酸可减少缺血诱导的 caspase-8 和 caspase-9 表达，这是 SON 和 PVN 细胞凋亡的关键诱导物。
Taurine is a sulphur-containing amino acid abundant in the nervous system. It protects cells from ischemia-induced apoptosis, but the mechanism underlying this is not well established. The aim of our study was to explore the effects of taurine on two main pathways of apoptosis induced by ischemia: receptor-mediated and mitochondrial cell death. Brain slices containing the supraoptic (SON) and paraventricular (PVN) nuclei of the hypothalamus were incubated in vitro under control and simulated ischemic (oxygen-glucose deprivation for 30 min) conditions in the absence and presence of 20 mM taurine. Brain slices were harvested after the 180-min “postischemic” period and fixed in 4% paraformaldehyde. To estimate apoptosis, immunostaining was done for caspase-8 and caspase-9 in paraffin-embedded sections. Immunoreactive caspase-8 and caspase-9 cells were observed in SON and PVN in all experimental groups, but in the “ischemic” group the expression of caspase-8 and caspase-9 and the number of immunoreactive cells was significantly increased in both hypothalamic nuclei. Addition of taurine (20 mM) to the incubation medium induced a marked decrease in caspase-8 and caspase-9 immunoreactivity after ischemia in SON and PVN when compared with the taurine-untreated “ischemic” group. Taurine reduces ischemia-induced caspase-8 and caspase-9 expression, the key inductors of apoptosis in SON and PVN.