Evolution of a tissue-specific splicing network.
Evolution of a tissue-specific splicing network.
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DOI：
10.1101/gad.2009011
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发表时间：
2011-03
影响因子：
10.5
通讯作者：
J. Matthew Taliaferro;Nehemiah S. Alvarez;Richard E. Green;M. Blanchette;D. C. Rio
中科院分区：
文献类型：
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作者：
J. Matthew Taliaferro;Nehemiah S. Alvarez;Richard E. Green;M. Blanchette;D. C. Rio
前体mRNA选择性剪接是大多数真核生物用来增加转录和蛋白质组多样性的一种策略。许多后生动物剪接因子是多基因家族的成员，每个成员具有不同的功能。这些高度相关的蛋白质如何进化出独特的性质尚不清楚。在这里，我们描述了一种新的果蝇剪接因子的进化和功能，称为LS2(大亚基2)，它源于dU2AF(50)的基因复制事件，dU2AF是高度保守的异二聚体通用剪接因子U2AF(U2相关因子)的大亚基。快速进化的LS2基因与剪接促进基因dU2AF(50)不同，它与明显不同的RNA序列结合，作为剪接抑制因子，并在睾丸中优先表达。LS2的目标转录本也被丰富，以执行与睾丸相关的功能。因此，我们提出了一条在果蝇中进化一种新的剪接因子的途径，这种剪接因子调节特定的前mRNAs，并以组织特异性的方式促进转录多样性。
Alternative splicing of precursor mRNA (pre-mRNA) is a strategy employed by most eukaryotes to increase transcript and proteomic diversity. Many metazoan splicing factors are members of multigene families, with each member having different functions. How these highly related proteins evolve unique properties has been unclear. Here we characterize the evolution and function of a new Drosophila splicing factor, termed LS2 (Large Subunit 2), that arose from a gene duplication event of dU2AF(50), the large subunit of the highly conserved heterodimeric general splicing factor U2AF (U2-associated factor). The quickly evolving LS2 gene has diverged from the splicing-promoting, ubiquitously expressed dU2AF(50) such that it binds a markedly different RNA sequence, acts as a splicing repressor, and is preferentially expressed in testes. Target transcripts of LS2 are also enriched for performing testes-related functions. We therefore propose a path for the evolution of a new splicing factor in Drosophila that regulates specific pre-mRNAs and contributes to transcript diversity in a tissue-specific manner.